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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Arabidopsis GLUTATHIONE REDUCTASE1 plays a crucial role in leaf responses to intracellular hydrogen peroxide and in ensuring appropriate gene expression through both salicylic acid and jasmonic acid signaling pathways
Plant Physiology, Volume 153, No. 3, Year 2010
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Description
Glutathione is a major cellular thiol that is maintained in the reduced state by glutathione reductase (GR), which is encoded by two genes in Arabidopsis (Arabidopsis thaliana; GR1 and GR2). This study addressed the role of GR1 in hydrogen peroxide (H2O2) responses through a combined genetic, transcriptomic, and redox profiling approach. To identify the potential role of changes in glutathione status in H2O2 signaling, gr1 mutants, which show a constitutive increase in oxidized glutathione (GSSG), were compared with a catalase-deficient background (cat2), in which GSSG accumulation is conditionally driven by H2O2. Parallel transcriptomics analysis of gr1 and cat2 identified overlapping gene expression profiles that in both lines were dependent on growth daylength. Overlapping genes included phytohormone-associated genes, in particular implicating glutathione oxidation state in the regulation of jasmonic acid signaling. Direct analysis of H2O2-glutathione interactions in cat2 gr1 double mutants established that GR1-dependent glutathione status is required for multiple responses to increased H2O2 availability, including limitation of lesion formation, accumulation of salicylic acid, induction of pathogenesis-related genes, and signaling through jasmonic acid pathways. Modulation of these responses in cat2 gr1 was linked to dramatic GSSG accumulation and modified expression of specific glutaredoxins and glutathione S-transferases, but there is little or no evidence of generalized oxidative stress or changes in thioredoxin-associated gene expression. We conclude that GR1 plays a crucial role in daylength-dependent redox signaling and that this function cannot be replaced by the second Arabidopsis GR gene or by thiol systems such as the thioredoxin system. © 2010 American Society of Plant Biologists.
Authors & Co-Authors
Mhamdi, Amna
Tunisia, Tunis
Université de Tunis el Manar, Faculté Des Sciences de Tunis
Hager, Jutta
France, Gif-sur-yvette
Institut Des Sciences Des Plantes de Paris-saclay
Chaouch, Sejir
France, Gif-sur-yvette
Institut Des Sciences Des Plantes de Paris-saclay
Queval, Guillaume
France, Gif-sur-yvette
Institut Des Sciences Des Plantes de Paris-saclay
Tunisia, Tunis
Université de Tunis el Manar, Faculté Des Sciences de Tunis
Belgium, Ghent
Universiteit Gent
Han, Yi
France, Gif-sur-yvette
Institut Des Sciences Des Plantes de Paris-saclay
Taconnat, Ludivine
France, Gif-sur-yvette
Institut Des Sciences Des Plantes de Paris-saclay
Saindrenan, Patrick
France, Gif-sur-yvette
Institut Des Sciences Des Plantes de Paris-saclay
Gouia, Houda
Tunisia, Tunis
Université de Tunis el Manar, Faculté Des Sciences de Tunis
Issakidis-Bourguet, Emmanuelle
France, Gif-sur-yvette
Institut Des Sciences Des Plantes de Paris-saclay
Renou, Jean Pierre
France, Gif-sur-yvette
Institut Des Sciences Des Plantes de Paris-saclay
Noctor, Graham D.
France, Gif-sur-yvette
Institut Des Sciences Des Plantes de Paris-saclay
Statistics
Citations: 304
Authors: 11
Affiliations: 3
Identifiers
Doi:
10.1104/pp.110.153767
ISSN:
00320889
e-ISSN:
15322548
Research Areas
Genetics And Genomics