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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Comparative genomic analysis of genogroup 1 and genogroup 2 rotaviruses circulating in seven US cities, 2014-2016
Virus Evolution, Volume 7, No. 1, Article veab023, Year 2021
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Description
For over a decade, the New Vaccine Surveillance Network (NVSN) has conducted active rotavirus (RVA) strain surveillance in the USA. The evolution of RVA in the post-vaccine introduction era and the possible effects of vaccine pressure on contemporary circulating strains in the USA are still under investigation. Here, we report the whole-gene characterization (eleven ORFs) for 157 RVA strains collected at seven NVSN sites during the 2014 through 2016 seasons. The sequenced strains included 52 G1P[8], 47 G12P[8], 18 G9P[8], 24 G2P[4], 5 G3P[6], as well as 7 vaccine strains, a single mixed strain (G9G12P[8]), and 3 less common strains. The majority of the single and mixed strains possessed a Wa-like backbone with consensus genotype constellation of G1/G3/G9/G12-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1, while the G2P[4], G3P[6], and G2P[8] strains displayed a DS-1-like genetic backbone with consensus constellation of G2/G3-P[4]/P[6]/P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2. Two intergenogroup reassortant G1P[8] strains were detected that appear to be progenies of reassortment events between Wa-like G1P[8] and DS-1-like G2P[4] strains. Two Rotarix® vaccine (RV1) and two RV5 derived (vd) reassortant strains were detected. Phylogenetic and similarity matrices analysis revealed 2-11 sub-genotypic allelic clusters among the genes of Wa- and DS-1-like strains. Most study strains clustered into previously defined alleles. Amino acid (AA) substitutions occurring in the neutralization epitopes of the VP7 and VP4 proteins characterized in this study were mostly neutral in nature, suggesting that these RVA proteins were possibly under strong negative or purifying selection in order to maintain competent and actual functionality, but fourteen radical (AA changes that occur between groups) AA substitutions were noted that may allow RVA strains to gain a selective advantage through immune escape. The tracking of RVA strains at the sub-genotypic allele constellation level will enhance our understanding of RVA evolution under vaccine pressure, help identify possible mechanisms of immune escape, and provide valuable information for formulation of future RVA vaccines. © 2021 Published by Oxford University Press 2021. This work is written by a US Government employee and is in the public domain in the US.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC8432945/bin/veab023_supplementary_data.xlsx
Authors & Co-Authors
Esona, Mathew Dioh
United States, Atlanta
Centers for Disease Control and Prevention
Gautam, Rashi
United States, Atlanta
Centers for Disease Control and Prevention
Katz, Eric M.
United States, Atlanta
Centers for Disease Control and Prevention
Betrapally, Naga S.
United States, Atlanta
Centers for Disease Control and Prevention
Selvarangan, Rangaraj
United States, Kansas City
Children's Mercy Hospitals and Clinics
Harrison, Christopher J.
United States, Kansas City
Children's Mercy Hospitals and Clinics
Klein, Eileen J.
United States, Seattle
Seattle Children's Hospital
McNeal, Monica Malone
United States, Cincinnati
Cincinnati Children's Hospital Medical Center
Halasa, Natasha B.
United States, Nashville
Vanderbilt University Medical Center
Chappell, James D.
United States, Nashville
Vanderbilt University Medical Center
Weinberg, Geoffrey A.
United States, Rochester
University of Rochester School of Medicine and Dentistry
Payne, Daniel C.
United States, Atlanta
Centers for Disease Control and Prevention
Parashar, Umesh D.
United States, Atlanta
Centers for Disease Control and Prevention
Bowen, Michael D.
United States, Atlanta
Centers for Disease Control and Prevention
Statistics
Citations: 13
Authors: 14
Affiliations: 7
Identifiers
Doi:
10.1093/ve/veab023
ISSN:
20571577
Research Areas
Genetics And Genomics