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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Comparison of transformation frequencies among selected Streptococcus pneumoniae serotypes
International Journal of Antimicrobial Agents, Volume 36, No. 2, Year 2010
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Description
Although there are over 90 serotypes of Streptococcus pneumoniae, antimicrobial resistance is predominantly found in a limited number of serotypes/serogroups, namely 6, 9, 14, 19 and 23. There is no compelling mechanism to account for this restriction. We aimed to determine whether serotypes commonly associated with drug resistance have higher transformation frequencies than those that are susceptible to antimicrobial agents. An in vitro investigation of the genetic transformation frequency of drug-resistant serotypes compared with that of susceptible serotypes under the influence of synthetic competence-stimulating peptides was performed. The transforming DNA was genomic DNA carrying a Tn. 916-like transposon containing the mefE gene that confers resistance to erythromycin. It was observed that serotypes 6, 9, 14, 19 and 23, which are highly associated with drug resistance, do not exhibit a higher degree of transformation efficiency than other serotypes. These findings suggest that the association of serotype with drug resistance is likely due to prolonged exposure to transforming DNA resulting from longer nasopharyngeal carriage and to a greater selective pressure from antimicrobials, particularly in children. This is the first study to compare the transformation frequencies of pneumococcal clinical isolates using genomic DNA that carries the composite Tn916-like element. © 2010 Elsevier B.V. and the International Society of Chemotherapy.
Authors & Co-Authors
Joloba, Moses Lutaakome
Uganda, Kampala
Makerere University College of Health Sciences
Kidenya, Benson Richard
Uganda, Kampala
Makerere University College of Health Sciences
Kateete, David Patrick
Uganda, Kampala
Makerere University College of Health Sciences
Katabazi, Fred Ashaba
Uganda, Kampala
Makerere University College of Health Sciences
Muwanguzi, Julian K.
Uganda, Kampala
Makerere University College of Health Sciences
Asiimwe, Benon Byamugisha
Uganda, Kampala
Makerere University College of Health Sciences
Alarakol, Simon Peter
Uganda, Kampala
Makerere University College of Health Sciences
Nakavuma, Jessica Lukanga
Uganda, Kampala
Makerere University
Bajaksouzian, Saralee
United States, Cleveland
Case Western Reserve University
United States, Cleveland
University Hospitals Case Medical Center
Windau, Anne R.
United States, Cleveland
Case Western Reserve University
United States, Cleveland
University Hospitals Case Medical Center
Jacobs, Michael R.
United States, Cleveland
Case Western Reserve University
United States, Cleveland
University Hospitals Case Medical Center
Statistics
Citations: 11
Authors: 11
Affiliations: 4
Identifiers
Doi:
10.1016/j.ijantimicag.2010.03.024
ISSN:
09248579
Research Areas
Cancer
Genetics And Genomics
Maternal And Child Health