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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Characterization of a unique human single-chain antibody isolated by phage-display selection on membrane-bound mosquito midgut antigens
Journal of Immunological Methods, Volume 261, No. 1-2, Year 2002
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Description
The insect midgut is the primary site for food digestion, as well as for vector-borne pathogen infection into the invertebrate host. Accordingly, antigens of this critical insect organ are targets for anti-vector vaccines, insecticidal toxins, and transmission-blocking vaccines. We used midgut proteins of the African malaria vector mosquito Anopheles gambiae to select single-chain human antibody fragments (scFv) from a high-diversity, phage-displayed library. Using a phage-display selection method on western-blotted antigens, we selected an unusual truncated scFv clone, consisting of a heavy-chain only, which binds to An. gambiae midgut tissue. This clone binds a spectrum of mosquito antigens from the midgut and other mosquito tissues, as well as various mammalian glycoproteins, but binding was reduced when these glycoproteins were enzymatically deglycosylated. We also observed that this clone preferentially binds the lumenal midgut surface. Furthermore, antigen binding by our selected scFv was limited by competition with increasing concentrations of certain soluble carbohydrates, most dramatically by galactose and N-acetyl glucosamine. Our results show that the cognate epitope of this scFv is a carbohydrate moiety. This paper describes a phage-display selection of antibody fragments on mosquito midgut tissue and it also describes a method for phage-display selection on membrane-immobilized heterogeneous antigens. These selection methods resulted in the isolation of a novel, truncated, carbohydrate-binding human antibody fragment from a naive phage-display library. © 2002 Elsevier Science B.V. All rights reserved.
Authors & Co-Authors
Foy, Brian D.
United States, New Orleans
Tulane University
United States, New Orleans
Tulane University School of Public Health and Tropical Medicine
Killeen, Gerry Francis
United States, New Orleans
Tulane University School of Public Health and Tropical Medicine
Kenya, Nairobi
International Centre of Insect Physiology and Ecology Nairobi
Frohn, Ross H.
United States, New Orleans
Tulane University School of Public Health and Tropical Medicine
Impoinvil, Daniel E.
United States, New Orleans
Tulane University School of Public Health and Tropical Medicine
Williams, Andrew J.
United Kingdom, Cambridge
Medimmune Limited, Cambridge
Beier, John C.
United States, New Orleans
Tulane University School of Public Health and Tropical Medicine
Statistics
Citations: 15
Authors: 6
Affiliations: 4
Identifiers
Doi:
10.1016/S0022-1759(01)00554-3
ISSN:
00221759
Research Areas
Food Security
Infectious Diseases