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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Reasons for false-positive lipoarabinomannan ELISA results in a Tanzanian population
Scandinavian Journal of Infectious Diseases, Volume 46, No. 2, Year 2014
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Description
Lipoarabinomannan (LAM), a cell wall component of mycobacteria, can be detected in the urine of tuberculosis (TB) patients. Advantages of this diagnostic include the ease of sample collection and test methods. However, as with most new TB diagnostics, LAM tests have been evaluated in well-controlled laboratory settings and subsequently need assessment under real working conditions. Our experience showed that the diagnosis of TB using the detection of LAM in urine under field conditions is prone to false-positive results due to contamination. Dust and soil, but also stool, seemed to lead to increased OD values and thus false-positive results of the enzyme-linked immunosorbent assay (ELISA) for LAM; however, contamination with blood, as well as bacterial or fungal organisms, had no influence. The collection of urine for the detection of LAM should therefore follow strict collection criteria in order to avoid contamination. © 2014 Informa Healthcare.
Authors & Co-Authors
Kroidl, Inge
Germany, Munich
Klinikum Der Universität München
Tanzania
Nimr-mbeya Medical Research Centre
Clowes, Petra
Tanzania
Nimr-mbeya Medical Research Centre
Mwakyelu, Joshua
Tanzania
Nimr-mbeya Medical Research Centre
Maboko, Leonard L.
Tanzania
Nimr-mbeya Medical Research Centre
Kiangi, Abubakary
Tanzania
Mbeya Referral Hospital Laboratory
Rachow, Andrea
Germany, Munich
Klinikum Der Universität München
Tanzania
Nimr-mbeya Medical Research Centre
Germany, Munich
German Center for Infection Research Dzif
Reither, Klaus
Tanzania
Nimr-mbeya Medical Research Centre
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Jung, Jutta
Tanzania
Nimr-mbeya Medical Research Centre
Nsojo, Anthony Ambikile
Tanzania
Nimr-mbeya Medical Research Centre
Saathoff, Elmar
Germany, Munich
Klinikum Der Universität München
Tanzania
Nimr-mbeya Medical Research Centre
Germany, Munich
German Center for Infection Research Dzif
Höelscher, Michael
Germany, Munich
Klinikum Der Universität München
Tanzania
Nimr-mbeya Medical Research Centre
Germany, Munich
German Center for Infection Research Dzif
Statistics
Citations: 27
Authors: 11
Affiliations: 5
Identifiers
Doi:
10.3109/00365548.2013.853133
ISSN:
00365548
Research Areas
Health System And Policy
Infectious Diseases
Study Design
Cross Sectional Study