Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Detection of the schistosome circulating cathodic antigen by enzyme immunoassay using biotinylated monoclonal antibodies
Transactions of the Royal Society of Tropical Medicine and Hygiene, Volume 84, No. 6, Year 1990
Notification
URL copied to clipboard!
Description
We have developed an enzyme immunoassay (EL-ISA) for the quantification of the schistosome circulating cathodic antigen (CCA), a glycoprotein associated with the syncitium lining the gut of the parasite. A mouse monoclonal antibody of IgG3 isotype was used as coating (antigen-capture) antibody, while a biotinylated mouse monoclonal IgM was used as second (antigen-detecting) antibody. Streptavidin-alkaline phosphatase was used as enzyme label. The lower detection limit of the assay was 1.0 ng of the trichloroacetic acid soluble fraction of adult worm antigen (AWA-TCA) per ml, which corresponds to approximately 0.2 ng CCA per ml. The ELISA showed a linear range from 1.0 to 62.5 ng AWA-TCA per ml. Serum and urine samples of 16 individuals infected with Schistosoma mansoni (egg counts ranging from 5 to 4820 eggs per gram of faeces) were tested in the assay. Antigen titres ranged from <4-8192. This assay represents a considerable advantage in diagnosis of Schistosoma infections as it allows the detection and quantification of CCA in serum and urine in even lightly infected individuals. © 1990, Oxford University Press. All rights reserved.
Authors & Co-Authors
de Jonge, Niels
Netherlands, Leiden
Universiteit Leiden
Kremsner, Peter G.
Germany, Berlin
Landesinstitut Für Tropenmedizin
Krijger, Frederik W.
Netherlands, Leiden
Universiteit Leiden
Schommer, Gabriele
Germany, Berlin
Landesinstitut Für Tropenmedizin
Kornelis, Dieuwke
Netherlands, Leiden
Universiteit Leiden
van Zeyl, René J.M.
Netherlands, Leiden
Universiteit Leiden
van Dam, Govert Jan
Netherlands, Leiden
Universiteit Leiden
Feldmeier, Hermann
Germany, Berlin
Landesinstitut Für Tropenmedizin
Deelder, André Martien
Netherlands, Leiden
Universiteit Leiden
Statistics
Citations: 85
Authors: 9
Affiliations: 2
Identifiers
Doi:
10.1016/0035-9203(90)90094-U
ISSN:
00359203