Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Genetic identification of endangered North African ungulates using noninvasive sampling
Molecular Ecology Resources, Volume 15, No. 3, Year 2015
Notification
URL copied to clipboard!
Description
North African ungulates include several threatened and emblematic species, yet are poorly studied mainly due to their remoteness and elusiveness. Noninvasive sampling provides a useful approach to obtain ecological and genetic information essential to guide conservation actions. The very first and most important step in conservation planning is to accurately identify species, and molecular genetics has been proved to be a useful tool. Several molecular genetics protocols are available for species identification, even for samples with poor quality DNA, such as faeces, hairs or bones. Most of these protocols use mitochondrial DNA for barcoding despite this marker being especially prone to problems, including mtDNA introgression, nuclear insert copies, high intraspecific diversity or heteroplasmy. In this work, we developed a molecular method based on polymorphisms in small fragments of the mitochondrial cytochrome b (cytb, mtDNA) and the nuclear kappa casein genes (KCAS, nDNA) for identifying endangered North African ungulates. These fragments revealed polymorphisms, including species-specific variation, which allowed species identification of nine ungulate species that co-occur in North Africa. The method was validated across more than 400 samples, including different types of noninvasive samples collected in the field. The simplicity, high reliability and relative low cost of the described method make it a promising tool to improve ecological studies of the North African ungulates and consequently, the implementation of more efficient management and conservation plans for these endangered ungulates. © 2014 John Wiley & Sons Ltd.
Authors & Co-Authors
Silva, Teresa Luísa
Portugal, Porto
Universidade do Porto
Spain, Madrid
Consejo Superior de Investigaciones Científicas
Godinho, Raquel
Portugal, Porto
Universidade do Porto
Castro, Diana
Portugal, Porto
Universidade do Porto
Abáigar, Teresa
Spain, Madrid
Consejo Superior de Investigaciones Científicas
Brito, J. C.
Portugal, Porto
Universidade do Porto
Alves, Paulo C.
Portugal, Porto
Universidade do Porto
United States, Missoula
University of Montana
Statistics
Citations: 26
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1111/1755-0998.12335
ISSN:
1755098X
Research Areas
Cancer
Genetics And Genomics
Study Locations
Multi-countries