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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Subtype-specific conservation of isoleucine 309 in the envelope V3 domain is linked to immune evasion in subtype C HIV-1 infection
Virology, Volume 404, No. 1, Year 2010
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Description
The V3 region of the HIV-1 envelope (Env) glycoprotein gp120 is a key functional domain yet it exhibits distinct mutational patterns across subtypes. Here an invariant residue (Ile 309) was replaced with Leu in 7 subtype C patient-derived Envs from recent infection and 4 related neutralizing antibody escape variants that emerged later. For these 11 Envs, I309L did not alter replication in primary CD4 T cells; however, replication in monocyte-derived macrophages was enhanced. Infection of cell lines with low CD4 or CCR5 revealed that I309L enhanced utilization of CD4 but did not affect the ability to use CCR5. This CD4-enhanced phenotype tracked with sensitivity to sCD4, indicating increased exposure of the CD4 binding site. The results suggest that Ile 309 preserves a V3-mediated masking function that occludes the CD4 binding site. The findings point to an immune evasion strategy in subtype C Env to protect this vulnerable immune target. © 2010 Elsevier Inc.
Authors & Co-Authors
Lynch, Rebecca M.
United States, Atlanta
Emory University
Rong, Rong
United States, Atlanta
Emory University
Li, Bing
United States, Atlanta
Emory University
Shen, Tongye
United States, Knoxville
The University of Tennessee, Knoxville
Honnen, William J.
United States, Newark
Public Health Research Institute
Mulenga, Joseph
Zambia, Lusaka
Zambia Blood Transfusion Service
Allen, Susan A.
United States, Atlanta
Emory University
United States, Atlanta
Rollins School of Public Health
Pinter, Abraham
United States, Newark
Public Health Research Institute
Gnanakaran, Sandrasegaram
United States, Los Alamos
Los Alamos National Laboratory Theoretical Division
Derdeyn, Cynthia A.
United States, Atlanta
Emory University
Statistics
Citations: 23
Authors: 10
Affiliations: 6
Identifiers
Doi:
10.1016/j.virol.2010.04.010
ISSN:
00426822
e-ISSN:
10960341
Research Areas
Health System And Policy
Infectious Diseases