Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Ragweed (Ambrosia artemisiifolia) pollen allergenicity: SuperSAGE transcriptomic analysis upon elevated CO
2
and drought stress
BMC Plant Biology, Volume 14, No. 1, Article 176, Year 2014
Notification
URL copied to clipboard!
Description
Background: Pollen of common ragweed (Ambrosia artemisiifolia) is a main cause of allergic diseases in Northern America. The weed has recently become spreading as a neophyte in Europe, while climate change may also affect the growth of the plant and additionally may also influence pollen allergenicity. To gain better insight in the molecular mechanisms in the development of ragweed pollen and its allergenic proteins under global change scenarios, we generated SuperSAGE libraries to identify differentially expressed transcripts.Results: Ragweed plants were grown in a greenhouse under 380 ppm CO2 and under elevated level of CO2 (700 ppm). In addition, drought experiments under both CO2 concentrations were performed. The pollen viability was not altered under elevated CO2, whereas drought stress decreased its viability. Increased levels of individual flavonoid metabolites were found under elevated CO2 and/or drought. Total RNA was isolated from ragweed pollen, exposed to the four mentioned scenarios and four SuperSAGE libraries were constructed. The library dataset included 236,942 unique sequences, showing overlapping as well as clear differently expressed sequence tags (ESTs). The analysis targeted ESTs known in Ambrosia, as well as in pollen of other plants. Among the identified ESTs, those encoding allergenic ragweed proteins (Amb a) increased under elevated CO2 and drought stress. In addition, ESTs encoding allergenic proteins in other plants were also identified.Conclusions: The analysis of changes in the transcriptome of ragweed pollen upon CO2 and drought stress using SuperSAGE indicates that under global change scenarios the pollen transcriptome was altered, and impacts the allergenic potential of ragweed pollen. © 2014 El Kelish et al.; licensee BioMed Central Ltd.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC4084800/bin/1471-2229-14-176-S1.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC4084800/bin/1471-2229-14-176-S2.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC4084800/bin/1471-2229-14-176-S3.zip
https://efashare.b-cdn.net/share/pmc/articles/PMC4084800/bin/1471-2229-14-176-S4.xls
https://efashare.b-cdn.net/share/pmc/articles/PMC4084800/bin/1471-2229-14-176-S5.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC4084800/bin/1471-2229-14-176-S6.xlsx
https://efashare.b-cdn.net/share/pmc/articles/PMC4084800/bin/1471-2229-14-176-S7.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC4084800/bin/1471-2229-14-176-S8.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC4084800/bin/1471-2229-14-176-S9.pdf
Authors & Co-Authors
Elkelish, Amr
Germany, Oberschleissheim
Helmholtz Center Munich German Research Center for Environmental Health
Egypt, Ismailia
Faculty of Science
Zhao, Feng
Germany, Oberschleissheim
Helmholtz Center Munich German Research Center for Environmental Health
Heller, Werner
Germany, Oberschleissheim
Helmholtz Center Munich German Research Center for Environmental Health
Durner, Jörg Ö.
Germany, Oberschleissheim
Helmholtz Center Munich German Research Center for Environmental Health
Germany, Munich
Technische Universität München
Winkler, Jana Barbro
Germany, Oberschleissheim
Helmholtz Center Munich German Research Center for Environmental Health
Behrendt, Heidrun
Germany, Oberschleissheim
Helmholtz Center Munich German Research Center for Environmental Health
Switzerland, Davos
Ck-care
Traidl-Hoffman, Claudia
Switzerland, Davos
Ck-care
Germany, Munich
Technische Universität München
Horres, Ralf
Germany, Frankfurt am Main
Genxpro Gmbh
Pfeifer, Matthias
Germany, Oberschleissheim
Helmholtz Center Munich German Research Center for Environmental Health
Frank, Ulrike
Germany, Oberschleissheim
Helmholtz Center Munich German Research Center for Environmental Health
Switzerland, Davos
Ck-care
Ernst, Dieter
Germany, Oberschleissheim
Helmholtz Center Munich German Research Center for Environmental Health
Switzerland, Davos
Ck-care
Statistics
Citations: 11
Authors: 11
Affiliations: 5
Identifiers
Doi:
10.1186/1471-2229-14-176
e-ISSN:
14712229
Research Areas
Environmental