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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Antibodies to proteins from yolk of immunized hens
Immunological Investigations, Volume 9, No. 5, Year 1980
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Description
Immunoglobulins IgY, extracted from yolks of hens immunized against several proteins and natural mixtures of proteins were examined by the Ouchterloney gel diffusion and Laurell immuno-electrophoresis techniques. The molecular weights of the proteins ranged from 8 × 106 to less than 2 × 104. The hens produced IgY antibodies readily when injected with the high molecular antigens >150 000 but did not react as well to antigens of relatively low molecular weight < 30 000. Optimal precipitin reactions were obtained with low molecular antigens when the agarose gel contained 1.5 M NaCl. Salt concentration (0.15-1.5 M NaCl) has no effect on the precipitin reaction in gels on the systems of high molecular antigens and their IgY antibodies apart from a displacement of the precipitin line. From the relative positions of the precipitin lines to the wells containing the reactants it would appear that the IgY formed dimers when the concentration of NaCl is 1.5 M and remain as monomers in gels containing 0.15 M NaCl. An explanation of the salt effect is offered. © 1980 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
Authors & Co-Authors
Polson, Alfred
South Africa, Cape Town
University of Cape Town
von Wechmar, M. Barbara
South Africa, Cape Town
University of Cape Town
Fazakerley, G.
South Africa, Cape Town
University of Cape Town
Statistics
Citations: 92
Authors: 3
Affiliations: 1
Identifiers
Doi:
10.3109/08820138009066011
ISSN:
08820139