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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Antibody specificities associated with neutralization breadth in plasma from human immunodeficiency virus type 1 subtype C-infected blood donors
Journal of Virology, Volume 83, No. 17, Year 2009
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Description
Defining the specificities of the anti-human immunodeficiency virus type 1 (HIV-1) envelope antibodies able to mediate broad heterologous neutralization will assist in identifying targets for an HIV-1 vaccine. We screened 70 plasmas from chronically HIV-1-infected individuals for neutralization breadth. Of these, 16 (23%) were found to neutralize 80% or more of the viruses tested. Anti-CD4 binding site (CD4bs) antibodies were found in almost all plasmas independent of their neutralization breadth, but they mainly mediated neutralization of the laboratory strain HxB2 with little effect on the primary virus, Du151. Adsorption with Du151 monomeric gp120 reduced neutralizing activity to some extent in most plasma samples when tested against the matched virus, although these antibodies did not always confer cross-neutralization. For one plasma, this activity was mapped to a site overlapping the CD4-induced (CD4i) epitope and CD4bs. Anti-membrane-proximal external region (MPER) (r = 0.69; P < 0.001) and anti-CD4i (r = 0.49; P < 0.001) antibody titers were found to be correlated with the neutralization breadth. These anti-MPER antibodies were not 4E10- or 2F5-like but spanned the 4E10 epitope. Furthermore, we found that anti-cardiolipin antibodies were correlated with the neutralization breadth (r = 0.67; P < 0.001) and anti-MPER antibodies (r = 0.6; P < 0.001). Our study suggests that more than one epitope on the envelope glycoprotein is involved in the cross-reactive neutralization elicited during natural HIV-1 infection, many of which are yet to be determined, and that polyreactive antibodies are possibly involved in this phenomenon. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC2738176/bin/supp_83_17_8925__index.html
https://efashare.b-cdn.net/share/pmc/articles/PMC2738176/bin/supp_83_17_8925__SupplementFigure1.ppt
https://efashare.b-cdn.net/share/pmc/articles/PMC2738176/bin/supp_83_17_8925__SupplementFigure2.ppt
https://efashare.b-cdn.net/share/pmc/articles/PMC2738176/bin/supp_83_17_8925__Legends.doc
https://efashare.b-cdn.net/share/pmc/articles/PMC2738176/bin/supp_83_17_8925__SupplementTable1.ppt
https://efashare.b-cdn.net/share/pmc/articles/PMC2738176/bin/supp_83_17_8925__SupplementTable2.ppt
Authors & Co-Authors
Gray, Elin Solomonovna
South Africa, Johannesburg
National Institute for Communicable Diseases
Taylor, Natasha
South Africa, Johannesburg
National Institute for Communicable Diseases
Wycuff, Diane R.
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Moore, Penny L.
South Africa, Johannesburg
National Institute for Communicable Diseases
Tomaras, Georgia D.
United States, Durham
Duke University Medical Center
Wibmer, Constantinos Kurt
South Africa, Johannesburg
National Institute for Communicable Diseases
Puren, Adrian
South Africa, Johannesburg
National Institute for Communicable Diseases
DeCamp, Allan C.
United States, Seattle
Fred Hutchinson Cancer Research Center
Gilbert, Peter B.
United States, Seattle
Fred Hutchinson Cancer Research Center
Wood, Blake
United States, Seattle
Fred Hutchinson Cancer Research Center
Montefiori, David Charles
United States, Durham
Duke University Medical Center
Binley, James M.
United States, San Diego
Torrey Pines Institute for Molecular Studies
Shaw, George M.
United States, Birmingham
The University of Alabama at Birmingham
Haynes, Barton F.
United States, Durham
Duke University Medical Center
Mascola, John R.
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Morris, Lynn
South Africa, Johannesburg
National Institute for Communicable Diseases
Statistics
Citations: 182
Authors: 16
Affiliations: 6
Identifiers
Doi:
10.1128/JVI.00758-09
ISSN:
0022538X
Research Areas
Infectious Diseases