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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Characterization of the immune response to Leishmania infantum recombinant antigens
Microbes and Infection, Volume 5, No. 1, Year 2003
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Description
Leishmaniases have a high prevalence in tropical countries. In order to improve existing diagnostic systems based on total Leishmania proteins, and to identify antigen candidates for vaccine development, an intensive search for the identification of antigens was performed using molecular biology techniques. In this study, the immune response to three L. infantum recombinant antigens was evaluated. Upon stimulation with KMP11, mononuclear cells from leishmaniasis patients produced high levels of IL-10, while a predominant IFN-γ production could be observed in cultures stimulated with H2A and soluble Leishmania antigen. All the recombinant antigens induced very little IL-5. KMP11 decreased IFN-γ production by 48% in cultures of peripheral blood mononuclear cells from cutaneous leishmaniasis patients who had been stimulated with soluble Leishmania antigen. Furthermore, antibodies to KMP11 were detected in the sera from all patients with visceral leishmaniasis and in the majority of the sera from patients with cutaneous leishmaniasis or individuals with asymptomatic L. chagasi infection. Thus, KMP11 is recognized by cells and sera of patients with different clinical forms of leishmaniasis, and KMP11, through IL-10 production, proved to be a potent antigen in modulating type 1 immune response. © 2003 Éditions scientifiques et médicales Elsevier SAS. All rights reserved.
Authors & Co-Authors
De Carvalho, Lucas Pedreira
Brazil, Salvador
Universidade Federal da Bahia
Soto, Manuel
Spain, Madrid
Csic-uam - Centro de Biología Molecular Severo Ochoa Cbm
Jeronimo, Selma M.B.
Brazil, Natal
Universidade Federal do Rio Grande do Norte
Dondji, Blaise
Cameroon, Ngaoundere
University of Ngaoundere
Bacellar, Olívia
Brazil, Salvador
Universidade Federal da Bahia
Luz, Viviane
Brazil, Natal
Universidade Federal do Rio Grande do Norte
Orge, Glória Orge
Brazil, Salvador
Universidade Federal da Bahia
Álonso, Carlos
Spain, Madrid
Csic-uam - Centro de Biología Molecular Severo Ochoa Cbm
Jesus, Amélia R.
Brazil, Salvador
Universidade Federal da Bahia
Carvalho, Edgar M.
Brazil, Salvador
Universidade Federal da Bahia
Statistics
Citations: 49
Authors: 10
Affiliations: 4
Identifiers
Doi:
10.1016/S1286-4579(02)00051-5
ISSN:
12864579
Study Design
Cross Sectional Study