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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Use of serum and blood samples on filter paper to improve the surveillance of dengue in Pacific Island Countries
Journal of Clinical Virology, Volume 55, No. 1, Year 2012
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Description
Background: In Pacific Island Countries (PICs) the epidemiology of dengue is characterized by long-term transmission of a single dengue virus (DENV) serotype. The emergence of a new serotype in one island country often indicates major outbreaks with this serotype will follow in other PICs. Objectives: Filter paper (FP) cards on which whole blood or serum from dengue suspected patients had been dried was evaluated as a method for transportation of this material by standard mail delivery throughout the Pacific. Study design: Twenty-two FP-dried whole blood samples collected from patients in New Caledonia and Wallis & Futuna Islands, during DENV-1 and DENV-4 transmission, and 76 FP-dried sera collected from patients in Yap State, Majuro (Republic of Marshall Islands), Tonga and Fiji, before and during outbreaks of DENV-2 in Yap State and DENV-4 in Majuro, were tested for the presence of DENV RNA, by serotype specific RT-PCR, at the Institut Louis Malardé in French Polynesia. Results: The serotype of DENV could be determined, by a variety of RT-PCR procedures, in the FP-dried samples after more than three weeks of transport at ambient temperatures. In most cases, the sequencing of the envelope gene to genotype the viruses also was possible. Conclusions: The serotype and genotype of DENV can be determined from FP-dried serum or whole blood samples transported over thousands of kilometers at ambient, tropical, temperatures. This simple and low-cost approach to virus identification should be evaluated in isolated and resource poor settings for surveillance for a range of significant viral diseases. © 2012.
Authors & Co-Authors
Dupont-Rouzeyrol, Myrielle
New Caledonia, Noumea
Institut Pasteur de Nouvelle-caledonie Noumea
Aaskov, John G.
Australia, Brisbane
Queensland University of Technology
Marfel, Maria
Unknown Affiliation
Lalita, Paul
Unknown Affiliation
Chanteau, Suzanne
New Caledonia, Noumea
Institut Pasteur de Nouvelle-caledonie Noumea
Musso, Didier
French Polynesia, Papeete
Institut Louis Malarde
Pavlin, Boris Igor
Switzerland, Geneva
Organisation Mondiale de la Santé
Kool, Jacob Leen
Switzerland, Geneva
Organisation Mondiale de la Santé
Cao-Lormeau, Van Mai
French Polynesia, Papeete
Institut Louis Malarde
Statistics
Citations: 31
Authors: 9
Affiliations: 6
Identifiers
Doi:
10.1016/j.jcv.2012.05.010
ISSN:
18735967
Research Areas
Genetics And Genomics
Infectious Diseases