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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Microsatellite analysis of Candida isolates from recurrent vulvovaginal candidiasis
Journal of Medical Microbiology, Volume 61, No. 8, Year 2012
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Description
Candida albicans and Candida glabrata are the most common causative agents of both vulvovaginal candidiasis (VVC) and recurrent vulvovaginal candidiasis (RVVC). Studying the population structure and genotype differentiation of Candida species that cause RVVC may lead to a significant improvement in clinical management. A total of 106 isolates were collected from 55 patients who were subdivided into three groups. Group I comprised 15 patients with RVVC (n550 isolates); group II comprised 16 patients, who had a history of at least two episodes of VVC in the last year (n=532 isolates, two from each patient); and group III comprised 24 patients (n=524 isolates) who had experienced a single episode of VVC in the previous 1 year period. C. albicans microsatellite markers CAI, CAIII and CAIV and C. glabrata RPM2, MTI and ERG3 microsatellites were amplified in a multiplex PCR. All isolates were subjected to population genetic analysis, which provided evidence that there is a predominantly clonal population structure of C. albicans in each group. However, recombination was detected to some degree in C. albicans isolates in group III. A genetic homogeneity between the different C. albicans groups was observed. Although, C. glabrata isolates showed an important genetic differentiation between group I and group III (F ST=50.207). Genotype analysis revealed that the dominant genotypes of C. glabrata and C. albicans strains were more prevalent in patients with RVVC. The frequent scenario for cases of recurrent infection in our study was strain replacement (53.3%). In conclusion, the identification of recurrence-associated genotypes and a specific C. glabrata population structure in the RVVC group could be a significant marker for further investigations of virulence factors and RVVC management. © 2012 SGM.
Authors & Co-Authors
Amouri, Imen Khlif
Tunisia, Sfax
Faculty of Medicine of Sfax
Sellami, Hayet
Tunisia, Sfax
Faculty of Medicine of Sfax
Abbes, Salma
Tunisia, Sfax
Faculty of Medicine of Sfax
Hadrich, Ines
Tunisia, Sfax
Faculty of Medicine of Sfax
Mahfoudh, Nadia
Tunisia, Sfax
Chu Hedi-chaker
Makni, Hafedh
Tunisia, Sfax
Chu Hedi-chaker
Ayadi, Ali
Tunisia, Sfax
Faculty of Medicine of Sfax
Statistics
Citations: 28
Authors: 7
Affiliations: 2
Identifiers
Doi:
10.1099/jmm.0.043992-0
ISSN:
00222615
Research Areas
Genetics And Genomics
Health System And Policy
Study Design
Cross Sectional Study