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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
A radioimmunoassay specific for [Gln
8
]LH-RH: Application in the confirmation of the structure of chicken hypothalamic luteinizing hormone-releasing hormone
Peptides, Volume 4, No. 6, Year 1983
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Description
In the first report on the chemical structure of a nonmammalian LH-RH, chicken hypothalamic LH-RH was demonstrated to be [Gln8]LH-RH [2-4]. However, these studies and subsequent reports [7,8] did not totally exclude the possibility of a reverse sequence of the two amino acids Leu-Gln. In view of the recently described structure of salmon brain LH-RH as [Trp7,Leu8]LH-RH [9], we undertook to confirm our earlier conclusion that chicken LH-RH is [Gln8]LH-RH and not [Gln7,Leu8]LH-RH. The immunologic, chromatographic and biological properties of natural chicken hypothalamic LH-RH were compared with those of the two synthetic peptides, [Gln8]LH-RH and [Gln7,Leu8]LH-RH. A radioimmunoassay highly specific for [Gln8]LH-RH was developed. Natural chicken LH-RH cross-reacted fully with the antiserum which requires the COOH-terminal Gln8 to Gly10-NH2 for binding, while [Gln7,Leu8]LH-RH showed less than 0.1% cross-reaction. On a high resolution reverse phase high performance liquid chromatography system, natural chicken LH-RH co-eluted with [Gln8]LH-RH and was well separated from [Gln7,Leu8]LH-RH. In a chicken anterior pituitary cell bioassay, natural chicken LH-RH and [Gln8]LH-RH were equipotent in stimulating luteinizing hormone release, while the relative potency of [Gln7,Leu8]LH-RH was 4.4%. These data, in particular the use of a specific [Gln8]LH-RH antiserum, provide conclusive evidence that chicken LH-RH is [Gln8]LH-RH. © 1983.
Authors & Co-Authors
King, Judy A.
South Africa, Cape Town
Faculty of Health Sciences
South Africa, Observatory
Groote Schuur Hospital
TOBLER, C.
South Africa, Cape Town
Faculty of Health Sciences
South Africa, Observatory
Groote Schuur Hospital
Roeske, Roger W.
United States, Indianapolis
Indiana University School of Medicine
Day, William A.
United States, Indianapolis
Indiana University School of Medicine
Rivier, Jean E.
United States, San Diego
Salk Institute for Biological Studies
Millar, Robert P.
South Africa, Cape Town
Faculty of Health Sciences
South Africa, Observatory
Groote Schuur Hospital
Statistics
Citations: 32
Authors: 6
Affiliations: 4
Identifiers
Doi:
10.1016/0196-9781(83)90086-4
ISSN:
01969781