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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Partial characterization of an exopolysaccharide secreted by a marine bacterium, vibrio neocaledonicus sp. nov., from new caledonia
Journal of Applied Microbiology, Volume 114, No. 6, Year 2013
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Description
Aims: Exopolysaccharides (EPS) are industrially valuable molecules with numerous useful properties. This study describes the techniques used for the identification of a novel Vibrio bacterium and preliminary characterization of its EPS. Methods and Results: Bioprospection in marine intertidal areas of New Caledonia followed by screening for EPS producing brought to selection of the isolate NC470. Phylogenetic analysis (biochemical tests, gene sequencing and DNA-DNA relatedness) permitted to identify NC470 as a new member of the Vibrio genus. The EPS was produced in batch fermentation, purified using the ultrafiltration process and analysed by colorimetry, Fourier Transform Infrared spectroscopy, gas chromatography, Nuclear Magnetic Resonance and HPLC-size exclusion chromatography. This EPS exhibits a high N-acetyl-hexosamines and uronic acid content with a low amount of neutral sugar. The molecular mass was 672 × 103 Da. These data are relevant for possible technological exploitation. Conclusions: We propose the name Vibrio neocaledonicus sp. nov for this isolate NC470, producing an EPS with an unusual sugar composition. Comparison with other known polymers permitted to select applications for this polymer. Significance and Impact of the Study: This study contributes to evaluate the marine biodiversity of New Caledonia. It also highlights the biotechnological potential of New Caledonia marine bacteria. © 2013 The Society for Applied Microbiology.
Authors & Co-Authors
Amir, Hamid
New Caledonia, Noumea
Universite de la Nouvelle-caledonie
Chanteau, Suzanne
New Caledonia, Noumea
Institut Pasteur de Nouvelle-caledonie Noumea
Dupont-Rouzeyrol, Myrielle
New Caledonia, Noumea
Institut Pasteur de Nouvelle-caledonie Noumea
Statistics
Citations: 23
Authors: 3
Affiliations: 4
Identifiers
Doi:
10.1111/jam.12184
ISSN:
13645072
Research Areas
Cancer
Genetics And Genomics