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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Vaccine potentials of an intrinsically unstructured fragment derived from the blood stage-associated Plasmodium falciparum protein PFF0165c
Infection and Immunity, Volume 77, No. 12, Year 2009
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Description
We have identified new malaria vaccine candidates through the combination of bioinformatics prediction of stable protein domains in the Plasmodium falciparum genome, chemical synthesis of polypeptides, in vitro biological functional assays, and association of an antigen-specific antibody response with protection against clinical malaria. Within the predicted open reading frame of P. falciparum hypothetical protein PFF0165c, several segments with low hydrophobic amino acid content, which are likely to be intrinsically unstructured, were identified. The synthetic peptide corresponding to one such segment (P27A) was well recognized by sera and peripheral blood mononuclear cells of adults living in different regions where malaria is endemic. High antibody titers were induced in different strains of mice and in rabbits immunized with the polypeptide formulated with different adjuvants. These antibodies recognized native epitopes in P. falciparum-infected erythrocytes, formed distinct bands in Western blots, and were inhibitory in an in vitro antibody-dependent cellular inhibition parasite-growth assay. The immunological properties of P27A, together with its low polymorphism and association with clinical protection from malaria in humans, warrant its further development as a malaria vaccine candidate. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Olugbile, Sope
Switzerland, Lausanne
Centre Hospitalier Universitaire Vaudois
Kulangara, Caroline
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Bang, Gilles
France, Paris
Institut Pasteur, Paris
Bertholet, S.
United States, Seattle
Infectious Disease Research Institute
Suzarte, Edith
Switzerland, Lausanne
Université de Lausanne Unil
Villard, Viviane
Switzerland, Lausanne
Université de Lausanne Unil
Frank, Geraldine
Switzerland, Lausanne
Université de Lausanne Unil
Audran, Régine
Switzerland, Lausanne
Centre Hospitalier Universitaire Vaudois
Razaname, A.
Switzerland, Lausanne
Université de Lausanne Unil
Nèbiè, Issa O.
Burkina Faso, Ouagadougou
Centre National de Recherche et de Formation Sur le Paludisme
Awobusuyi, Olugbenga
Nigeria, Lagos
Lagos State University Teaching Hospital
Spertini, François
Switzerland, Lausanne
Centre Hospitalier Universitaire Vaudois
Kajava, Andrey V.
France, Montpellier
Centre de Recherche en Biologie Cellulaire de Montpellier
Felger, Ingrid
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Druilhe, Pierre L.
France, Paris
Institut Pasteur, Paris
Corradin, Giampietro
Switzerland, Lausanne
Université de Lausanne Unil
Statistics
Citations: 58
Authors: 16
Affiliations: 8
Identifiers
Doi:
10.1128/IAI.00652-09
ISSN:
00199567
e-ISSN:
10985522
Research Areas
Infectious Diseases