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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Typing of human rotaviruses: Nucleotide mismatches between the VP7 gene and primer are associated with genotyping failure
Virology Journal, Volume 2, Article 24, Year 2005
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Description
Background: Rotavirus genotyping is performed by using reverse transcription PCR with type-specific-primers. Because the high rotavirus mutation rate generates an extensive genomic variation, different G-type-specific primer sets are applied in different geographical locations. In Bangladesh, a significant proportion (36.9%) of the rotavirus strains isolated in 2002 could not be G-typed using the routinely used primer set. To investigate the reason why the strains were untypeable, nucleotide sequencing of the VP7 genes was performed. Results: Four nucleotide substitutions at the G1 primer-binding site of the VP7 gene of Bangladeshi G1 rotaviruses rendered a major proportion of circulating strains untypeable using the routine primer set. Using an alternative primer set, we could identify G1 rotaviruses as the most prevalent genotype (44.8%), followed by G9 (21.7%), G2 (15.0%) and G4 (13.8%). Conclusion: Because of the natural variation in the rotaviral gene sequences, close monitoring of rotavirus genotyping methods is important. © 2005 Rahman et al; licensee BioMed Central Ltd.
Authors & Co-Authors
Rahman, Mustafizur Mizanur Hafizur
Bangladesh, Dhaka
International Centre for Diarrhoeal Disease Research Bangladesh
Belgium, Leuven
Ku Leuven
Podder, Goutam
Bangladesh, Dhaka
International Centre for Diarrhoeal Disease Research Bangladesh
Faruque, Abu Syed Golam
Bangladesh, Dhaka
International Centre for Diarrhoeal Disease Research Bangladesh
Matthijnssens, Jelle
Belgium, Leuven
Ku Leuven
Breiman, Robert F.
Bangladesh, Dhaka
International Centre for Diarrhoeal Disease Research Bangladesh
Sack, David Allen
Bangladesh, Dhaka
International Centre for Diarrhoeal Disease Research Bangladesh
van Ranst, Marc A.
Belgium, Leuven
Ku Leuven
Azim, Tasnim
Bangladesh, Dhaka
International Centre for Diarrhoeal Disease Research Bangladesh
Statistics
Citations: 33
Authors: 8
Affiliations: 2
Identifiers
Doi:
10.1186/1743-422X-2-24
ISSN:
1743422X
Research Areas
Cancer
Genetics And Genomics