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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Effect of grain structure and cooking on sorghum and maize in vitro protein digestibility
Journal of Cereal Science, Volume 35, No. 2, Year 2002
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Description
Uncooked and cooked sorghum showed improvement in in vitro protein digestibility as the structural complexity of the sample reduced from whole grain flour through endosperm flour to protein body-enriched samples. This was not the case for maize. Cooking reduced protein digestibility of sorghum but not maize. Treating cooked sorghum and maize whole grain and endosperm flours with alpha-amylase to reduce sample complexity before in vitro pepsin digestion slightly improved protein digestibility. The reduction in sorghum protein digestibility on cooking was not related to the total polyphenol content of samples. Pericarp components, germ, endosperm cell walls, and gelatinised starch were identified as possible factors limiting sorghum protein digestibility. Electrophoresis of uncooked and cooked protein-body-enriched samples of sorghum and maize, and prolamin fractions of sorghum under non-reducing conditions showed oligomeric proteins with molecular weights (Mr) 45, 66 and >66 kDa and monomeric kafirins and zeins. Protein-body-enriched samples of sorghum had more 45-50 kDa oligomers than those of maize. In cooked sorghum, some of these were resistant to reduction. Pepsin-indigestible residues from protein-body-enriched samples consisted mainly of α-zein (uncooked and cooked maize) or α-kafirin (uncooked sorghum), whilst cooked sorghum had in addition, β- and γ-kafirin and reduction-resistant 45-50 kDa oligomers. Cooking appears to lead to formation of disulphide-bonded oligomeric proteins that occurs to a greater extent in sorghum than in maize. This may explain the poorer protein digestibility of cooked sorghum. © 2002 Elsevier Science Ltd.
Authors & Co-Authors
Duodu, K. G.
South Africa, Pretoria
University of Pretoria
Nunes, A.
Unknown Affiliation
Delgadillo, I.
Portugal, Aveiro
Universidade de Aveiro
Parker, Mary L.
United Kingdom, Norwich
Norwich Research Park
Mills, Elisabeth Noami Clare
United Kingdom, Norwich
Norwich Research Park
Belton, Peter S.
United Kingdom, Norwich
Norwich Research Park
Taylor, John R.N.
South Africa, Pretoria
University of Pretoria
Statistics
Citations: 224
Authors: 7
Affiliations: 3
Identifiers
Doi:
10.1006/jcrs.2001.0411