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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
pharmacology, toxicology and pharmaceutics
Two purified and characterized phospholipases A2 from Cerastes cerastes venom, that inhibit cancerous cell adhesion and migration
Toxicon, Volume 53, No. 4, Year 2009
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Description
Two non-toxic PLA2s were purified to homogeneity from Cerastes cerastes Tunisian snake venom. The purification process employed gel filtration on Sephadex G-75 followed by C18 reverse phase high-pressure liquid chromatography. These two acidic enzymes, namely CC-PLA2-1 and CC-PLA2-2, have a molecular weight of 13,737.52 and 13,705.63 Da, respectively. These two PLA2 are the first reported glycosylated phospholipases A2 purified from snake venom. The rates of glycosylation are 2.5% and 0.5% (w/w), respectively. Specific activities of 1800 U/mg and 2400 U/mg for CC-PLA2-1 and CC-PLA2-2, respectively, were measured at optimal conditions. CC-PLA2-1 and CC-PLA2-2 strongly inhibited coagulation. They also exhibited a marked dose-dependent inhibitory effect on platelet aggregation induced by ADP and arachidonic acid in platelet-rich plasma. Interestingly, CC-PLA2-1 and CC-PLA2-2 inhibited in a dose-dependent manner adhesion of IGR39 melanoma and HT1080 fibrosarcoma cells to fibrinogen and fibronectin. Furthermore, both CC-PLA2-1 and CC-PLA2-2 abolished HT1080 cell migration towards fibrinogen and fibronectin. This activity is reported for the first time for PLA2 enzymes. © 2009 Elsevier Ltd. All rights reserved.
Authors & Co-Authors
Zouari-Kessentini, Raoudha
Tunisia, Tunis
Institut Pasteur de Tunis
Luis, José
France, Beziers
Centre de Recherche en Oncologie Biologique et Onco-pharmacologie
Karray, Aida
Tunisia, Sfax
University of Sfax
Kallech-Ziri, Olfa
Tunisia, Tunis
Institut Pasteur de Tunis
Srairi-Abid, Najet
Tunisia, Tunis
Institut Pasteur de Tunis
Bazaa, Amine
Tunisia, Tunis
Institut Pasteur de Tunis
Loret, Erwann P.
France, Beziers
Centre de Recherche en Oncologie Biologique et Onco-pharmacologie
Bezzine, Sofiane
Tunisia, Sfax
University of Sfax
El-Ayeb, Mohamed E.
Tunisia, Tunis
Institut Pasteur de Tunis
Marrakchi, Naziha
Tunisia, Tunis
Institut Pasteur de Tunis
Tunisia, Tunis
Faculté de Médecine de Tunis
Statistics
Citations: 55
Authors: 10
Affiliations: 4
Identifiers
Doi:
10.1016/j.toxicon.2009.01.003
ISSN:
00410101
Research Areas
Cancer
Environmental