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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Mycobacteria causing human cervical lymphadenitis in pastoral communities in the Karamoja region of Uganda
Epidemiology and Infection, Volume 136, No. 5, Year 2008
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Description
Mycobacteria from lymph node biopsies of patients with cervical lymphadenitis reporting for tuberculosis treatment in Matany and Moroto Hospitals in the transhumant areas of Karamoja, Uganda were isolated and characterized. The AccuProbe® culture identification kits for Mycobacterium tuberculosis complex (MTC), M. avium complex (MAC) and M. avium were used to identify the isolates. Spoligotyping, IS 901 PCR and IS 1311 and IS 1245 restriction fragment length polymorphism (RFLP) were used to characterize the isolates. Of the 43 biopsies, ten M. avium, seven M. tuberculosis, three M. bovis, and two M. intracellulare were isolated. Two isolates could not be identified with AccuProbe® and from 19 samples no mycobacteria could be isolated. Three isolates with the Beijing spoligotype were identified from the seven M. tuberculosis isolates. The spoligopatterns of the M. bovis isolates had previously been detected in cattle in Uganda. Isolation of members of the MAC group reflects the complex interaction between the transhumant communities, water sources and their cattle. None of the M. avium isolates harboured IS 901, and all showed several bands on IS 1311 and IS 1245 RFLP, in accordance with M. avium subsp. hominissuis. Composite dendrograms of IS 1311 and IS 1245 RFLP showed that the isolates were similar and identical patterns were found. The isolation of M. bovis confirms the human infection with zoonotic mycobacteria in areas where consumption of raw milk and meat is routine. Isolation of environmental mycobacteria also confirms their increasing role in human disease and the occupational risk of infection in the transhumant ecosystem in the absence of safe drinking water and environmental contamination. © 2007 Cambridge University Press.
Authors & Co-Authors
Oloya, James
Uganda, Kampala
Makerere University
Norway, As
Norges Miljø- og Biovitenskapelige Universitet
Opuda-Asibo, John
Uganda, Kampala
Makerere University
Kazwala, Rudovick Reuben
Tanzania, Morogoro
Sokoine University of Agriculture
Demelash, A. B.
Ethiopia, Awassa
Debub University
Skjerve, Eystein
Norway, As
Norges Miljø- og Biovitenskapelige Universitet
Lund, Arve
Norway, Oslo
Veterinærinstituttet
Johansen, Tone Bjordal
Norway, Oslo
Veterinærinstituttet
DjØnne, Berit Kristoffersen
Norway, Oslo
Veterinærinstituttet
Statistics
Citations: 77
Authors: 8
Affiliations: 5
Identifiers
Doi:
10.1017/S0950268807009004
ISSN:
09502688
e-ISSN:
14694409
Research Areas
Environmental
Infectious Diseases
Study Locations
Uganda