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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Exploring proteins in Anopheles gambiae male and female antennae through MALDI mass spectrometry profiling
PLoS ONE, Volume 3, No. 7, Article e2822, Year 2008
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Description
MALDI profiling and imaging mass spectrometry (IMS) are novel techniques for direct analysis of peptides and small proteins in biological tissues. In this work we applied them to the study of Anopheles gambiae antennae, with the aim of analysing expression of soluble proteins involved in olfaction perireceptor events. MALDI spectra obtained by direct profiling on single antennae and by the analysis of extracts, showed similar profiles, although spectra obtained through profiling had a richer ion population and higher signal to noise ratio. Male and female antennae showed distinct protein profiles. MALDI imaging experiments were also performed and differences were observed in the localization of some proteins. Two proteins were identified through high resolution measurement and top-down MS/MS experiments. A 8 kDa protein only present in the male antennae matched with an unannotated sequence of the An. gambiae genome, while the presence of odorant binding protein 9 (OBP-9) was confirmed through experiments of 2-DE, followed by MS and MS/MS analysis of digested spots. This work shows that MALDI MS profiling is a technique suitable for the analysis of proteins of small and medium MW in insect appendices, and allows obtaining data for several specimens which can be investigated for differences between groups. Proteins of interest can be identified through other complementary MS approaches. © 2008 Dani et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC2474704/bin/pone.0002822.s001.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC2474704/bin/pone.0002822.s002.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC2474704/bin/pone.0002822.s003.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC2474704/bin/pone.0002822.s004.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC2474704/bin/pone.0002822.s005.doc
Authors & Co-Authors
Dani, Francesca Romana
Italy, Florence
Università Degli Studi Di Firenze
Francese, Simona
Italy, Florence
Università Degli Studi Di Firenze
Mastrobuoni, Guido
Italy, Florence
Università Degli Studi Di Firenze
Felicioli, Antonio
Italy, Pisa
Università Di Pisa
Caputo, Beniamino
Italy, Rome
Sapienza Università Di Roma
Simard, Frédéric R.
France, Montpellier
Ird Centre de Montpellier
Cameroon, Yaounde
Organisation de Coordination Pour la Lutte Contre Les Endémies en Afrique Centrale
Pieraccini, Giuseppe
Italy, Florence
Università Degli Studi Di Firenze
Moneti, Gloriano
Italy, Florence
Università Degli Studi Di Firenze
Coluzzi, Mario Luisa
Italy, Rome
Sapienza Università Di Roma
della Torre, Alessandra
Italy, Rome
Sapienza Università Di Roma
Turillazzi, S.
Italy, Florence
Università Degli Studi Di Firenze
Statistics
Citations: 26
Authors: 11
Affiliations: 5
Identifiers
Doi:
10.1371/journal.pone.0002822
e-ISSN:
19326203
Study Design
Cross Sectional Study
Participants Gender
Male
Female