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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Characterization of a stem cell population in lung cancer A549 cells
Biochemical and Biophysical Research Communications, Volume 371, No. 1, Year 2008
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Description
We isolated a stem cell subpopulation from human lung cancer A549 cells using FACS/Hoechst 33342. This side population (SP), which comprised 24% of the total cell population, totally disappeared after treatment with the selective ABCG 2 inhibitor fumitremorgin C. In a repopulation study, isolated SP and non-SP cells were each able to generate a heterogeneous population of SP and non-SP cells, but this repopulation occurred more rapidly in SP cells than non-SP. An MTT assay and cell cycle distribution analysis reveal a similar profile between SP and non-SP groups. However, in the presence of doxorubicin (DOX) and methotrexate (MTX), SP cells showed significantly lower Annexin V staining when compared to non-SP cells. Taken together, these results demonstrate that SP cells have an active regeneration capacity and high anti-apoptotic activity compared with non-SP cells. Furthermore, our GeneChip® data revealed a heightened mRNA expression of ABCG2 and ABCC2 in SP cells. Overall these data explain why the SP of A549 has a unique ability to resist DOX and MTX treatments. Therefore, we suggest that the expression of the ABCG2 transporter plays an important role in the multidrug resistance phenotype of A549 SP cells. © 2008 Elsevier Inc. All rights reserved.
Authors & Co-Authors
Sung, Ji-min
South Korea, Seoul
Konkuk University
Cho, Hee-jung
South Korea, Seoul
Konkuk University
Yi, Hee
South Korea, Seoul
Konkuk University
Lee, Chiho
South Korea, Seoul
Konkuk University
Abd El-Aty, A. M.
South Korea, Seoul
Konkuk University
Kim, Jin-suk
South Korea, Seoul
Konkuk University
Landowski, Christopher P.
Switzerland, Bern
University of Bern
Shin, Ho-chul
South Korea, Seoul
Konkuk University
Statistics
Citations: 114
Authors: 8
Affiliations: 3
Identifiers
Doi:
10.1016/j.bbrc.2008.04.038
ISSN:
10902104
Research Areas
Cancer
Study Design
Cross Sectional Study