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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Persistent infection of thymic epithelial cells with coxsackievirus B4 results in decreased expression of type 2 insulin-like growth factor
Journal of Virology, Volume 86, No. 20, Year 2012
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Description
It has been hypothesized that a disturbance of central self-tolerance to islet β cells may play a role in the enteroviral pathogenesis of type 1 diabetes. Whether enteroviruses can induce an impaired expression of β-cell self-antigens in thymic epithelial cells has been investigated in a murine thymic epithelial (MTE) cell line. This cell line was permissive to the diabetogenic group B4 coxsackievirus (CV-B4) strain CV-B4 E2 and spontaneously expressed type 2 insulin-like growth factor (Igf2), the dominant selfantigen of the insulin family. In this model, a persistent replication of CV-B4 E2 was obtained, as attested to by the prolonged detection of intracellular positive- and negative-strand viral RNA by reverse transcription-PCR (RT-PCR) and capsid protein VP1 by immunofluorescent staining and by the release of infectious particles in culture supernatants. The chronic stage of the infection was characterized by a low proportion of VP1-positive cells (1 to 2%), whereas many cells harbored enteroviral RNA, as displayed by RT-PCR without extraction applied directly to a few cells. Igf2 mRNA and IGF-2 protein were dramatically decreased in CV-B4 E2-infected MTE cell cultures compared with mock-infected cultures, whereas housekeeping and interleukin-6 (Il6) gene expression was maintained and Igf1 mRNA was decreased, but to a lower extent. Inoculation of CV-B3, CV-B4 JVB, or echovirus 1 resulted in a low level of IGF-2 in culture supernatants as well, whereas herpes simplex virus 1 stimulated the production of the protein. Thus, a persistent infection of a thymic epithelial cell line with enteroviruses like CV-B4 E2 can result in a disturbed production of IGF-2, a protein involved in central self-tolerance toward islet β cells. © 2012, American Society for Microbiology.
Authors & Co-Authors
Jaïdane, Hela
France, Lille
Laboratoire de Virologie
Tunisia, Monastir
Faculté de Pharmacie de Monastir
Tunisia, Tunis
Université de Tunis el Manar, Faculté Des Sciences de Tunis
Caloone, Delphine
France, Lille
Laboratoire de Virologie
Lobert, Pierre Emmanuel
France, Lille
Laboratoire de Virologie
Sane, F.
France, Lille
Laboratoire de Virologie
Dardenne, Olivier
Belgium, Liege
Université de Liège
Naquet, Philippe
France, Marseille
Centre D’immunologie de Marseille-luminy
Gharbi, Jawhar
Tunisia, Monastir
Faculté de Pharmacie de Monastir
Aouni, Mahjoub
Tunisia, Monastir
Faculté de Pharmacie de Monastir
Geenen, Vincent
Belgium, Liege
Université de Liège
Hober, Didier
France, Lille
Laboratoire de Virologie
Statistics
Citations: 44
Authors: 10
Affiliations: 5
Identifiers
Doi:
10.1128/JVI.00726-12
ISSN:
0022538X
e-ISSN:
10985514
Research Areas
Genetics And Genomics
Noncommunicable Diseases