Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Development of a set of chromosome segment substitution lines in pearl millet [Pennisetum glaucum (L.) R. Br.]
Crop Science, Volume 54, No. 5, Year 2014
Notification
URL copied to clipboard!
Description
The detection of minor quantitative trait loci (QTL) with conventional mapping populations can be complicated by the overshadowing effect of major QTL as well as by interactions between QTL. To overcome these constraints, we developed a set of chromosome segment substitution lines (CSSLs) by introgression of overlapping chromosome segments from 863B into ICMB 841 background for use in QTL detection, fine mapping, and trait mechanism studies, especially for complex traits. Since each CSSL carries one or a few donor segments in the genetic background of the recurrent genotype, the QTL interaction is confined to genes present on small homozygous substituted segments. Advanced generation backcross progenies (1492), expected to provide coverage across the mapped length of each of the seven pearl millet linkage groups (LGs), were genotyped at 74 marker loci [(48 simple sequence repeats (SSRs), 21 single strand conformation polymorphism-single nucleotide polymorphism (SSCP-SNP), and 5 sequence tagged sites (STSs)] identifying 124 segment introgression homozygotes (13 for LG1, 9 for LG2, 10 for LG3, 41 for LG4, 23 for LG5, 11 for LG6, and 17 for LG7). These CSSLs consisted of 1-3 homozygous introgression segments substituted from 863B in the genetic background of the recurrent parent ICMB 841 and among them, 54 represent unique lines with the donor chromosome segment averaging 100.69 cM. These CSSLs developed here provide a nearly ideal set of genetic stocks for mapping and fine mapping the multitude of traits for which their parents differ. © Crop Science Society of America.
Authors & Co-Authors
Ramana Kumari, Basava
India, Patancheru
International Crops Research Institute for the Semi-arid Tropics
Kolesnikova-Allen, Maria A.
United Kingdom, Hertford
Tun Abdul Razak Research Centre
Hash, Charles Tom
India, Patancheru
International Crops Research Institute for the Semi-arid Tropics
Senthilvel, Senapathy
India, Patancheru
International Crops Research Institute for the Semi-arid Tropics
Thirunavukkarasu, Nepolean
India, Patancheru
International Crops Research Institute for the Semi-arid Tropics
Kavi Kishor, Polavarapu Bilhan
India, Hyderabad
Osmania University
Riera-Lizarazu, Oscar
India, Patancheru
International Crops Research Institute for the Semi-arid Tropics
Witcombe, John R.
United Kingdom, Bangor
Bangor University
Srivastava, Rakesh Kumar
India, Patancheru
International Crops Research Institute for the Semi-arid Tropics
Statistics
Citations: 10
Authors: 9
Affiliations: 4
Identifiers
Doi:
10.2135/cropsci2013.09.0589
ISSN:
0011183X
Research Areas
Genetics And Genomics
Health System And Policy
Study Approach
Quantitative