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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Homoeologous chromosome pairing between the A and B genomes of Musa spp. revealed by genomic in situ hybridization
Annals of Botany, Volume 108, No. 5, Year 2011
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Description
Background and AimsMost cooking banana and several desert bananas are interspecific triploid hybrids between Musa acuminata (A genome) and Musa balbisiana (B genome). In addition, M. balbisiana has agronomical characteristics such as resistance to biotic and abiotic stresses that could be useful to improve monospecific acuminata cultivars. To develop efficient breeding strategies for improving Musa cultivars, it is therefore important to understand the possibility of chromosome exchange between these two species.MethodsA protocol was developed to prepare chromosome at meiosis metaphase I suitable for genomic in situ hybridization. A series of technical challenges were encountered, the main ones being the hardness of the cell wall and the density of the microsporocytes cytoplasm, which hampers accessibility of the probes to the chromosomes. Key parameters in solving these problems were addition of macerozyme in the enzyme mix, the duration of digestion and temperature during the spreading phase.Results and ConclusionsThis method was applied to analyse chromosome pairing in metaphase from triploid interspecific cultivars, and it was clearly demonstrated that interspecific recombinations between M. acuminata and M. balbisiana chromosomes do occur and may be frequent in triploid hybrids. These results provide new insight into Musa cultivar evolution and have important implications for breeding. © The Author 2011.
Authors & Co-Authors
Jeridi, Mouna
France, Montpellier
Amélioration Génétique et Adaptation Des Plantes Méditerranéennes et Tropicales Agap
Tunisia, Medenine
Institut Des Régions Arides
Bakry, Frédéric
France, Montpellier
Amélioration Génétique et Adaptation Des Plantes Méditerranéennes et Tropicales Agap
Escoute, Jacques
France, Montpellier
Amélioration Génétique et Adaptation Des Plantes Méditerranéennes et Tropicales Agap
Fondi, Emmanuel
Cameroon, Douala
Centre Africain de Recherche Sur Bananiers et Plantains Carbap
Carreel, Franoise
France, Montpellier
Amélioration Génétique et Adaptation Des Plantes Méditerranéennes et Tropicales Agap
Ferchichi, Ali
Tunisia, Medenine
Institut Des Régions Arides
D'Hont, Angélique
France, Montpellier
Amélioration Génétique et Adaptation Des Plantes Méditerranéennes et Tropicales Agap
Rodier-Goud, Marguerite
France, Montpellier
Amélioration Génétique et Adaptation Des Plantes Méditerranéennes et Tropicales Agap
Statistics
Citations: 41
Authors: 8
Affiliations: 3
Identifiers
Doi:
10.1093/aob/mcr207
ISSN:
03057364
Research Areas
Genetics And Genomics