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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Combination of cytokine responses indicative of latent TB and active TB in malawian adults
PLoS ONE, Volume 8, No. 11, Article e79742, Year 2013
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Description
Background: An IFN-γ response to M. tuberculosis -specific antigens is an effective biomarker for M. tuberculosis infection but it cannot discriminate between latent TB infection and active TB disease. Combining a number of cytokine/chemokine responses to M. tuberculosis antigens may enable differentiation of latent TB from active disease. Methods: Asymptomatic recently-exposed individuals (spouses of TB patients) were recruited and tuberculin skin tested, bled and followed-up for two years. Culture supernatants, from a six-day culture of diluted whole blood samples stimulated with M. tuberculosis -derived PPD or ESAT-6, were measured for IFN-γ, IL-10, IL-13, IL-17, TNF-α and CXCL10 using cytokine ELISAs. In addition, 15 patients with sputum smear-positive pulmonary TB were recruited and tested. Results: Spouses with positive IFN-γ responses to M. tuberculosis ESAT-6 (>62.5 pg/mL) and TB patients showed high production of IL-17, CXCL10 and TNF-α. Higher production of IL-10 and IL-17 in response to ESAT-6 was observed in the spouses compared with TB patients while the ratios of IFN-γ/IL-10 and IFN-γ/IL-17 in response to M. tuberculosis-derived PPD were significantly higher in TB patients compared with the spouses. Tuberculin skin test results did not correlate with cytokine responses. Conclusions: CXCL10 and TNF-α may be used as adjunct markers alongside an IFN-γ release assay to diagnose M. tuberculosis infection, and IL-17 and IL-10 production may differentiate individuals with LTBI from active TB. © 2013 Hur et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3832606/bin/pone.0079742.s001.tif
Authors & Co-Authors
Hur, Yun-gyoung
Unknown Affiliation
Gorak-Stolinska, Patricia M.A.
Unknown Affiliation
Ben-Smith, Anne W.
Unknown Affiliation
Lalor, Maeve K.
Unknown Affiliation
Chaguluka, Steven D.
Unknown Affiliation
Dacombe, Russell J.
Unknown Affiliation
Doherty, Timothy Mark
Unknown Affiliation
Ottenhoff, Tom H.M.
Unknown Affiliation
Dockrell, Hazel M.
Unknown Affiliation
Crampin, Amelia Catharine
Unknown Affiliation
Statistics
Citations: 44
Authors: 10
Affiliations: 5
Identifiers
Doi:
10.1371/journal.pone.0079742
e-ISSN:
19326203
Research Areas
Infectious Diseases