Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Rapid microarray-based method for monitoring of all currently known single-nucleotide polymorphisms associated with parasite resistance to antimalaria drugs
Journal of Clinical Microbiology, Volume 45, No. 11, Year 2007
Notification
URL copied to clipboard!
Description
Parasite drug resistance is partly conferred by single-nucleotide polymorphisms (SNPs), and monitoring them has been proposed as an alternative to monitoring drug resistance. Therefore, techniques are required to facilitate analyses of multiple SNPs on an epidemiological scale. We report a rapid and affordable microarray technique for application in epidemiological studies of malaria drug resistance. We have designed a multiwell microarray that is used in conjunction with PCR-amplified target genes implicated in the drug resistance of malaria with subsequent one-tube minisequencing using two fluorochromes. The drug-resistance-associated genes pfdhfr, pfdhps, pfcrt, pfmdr1, and pfATPase were amplified and analyzed for cultured Plasmodium falciparum strains and from field samples. We obtained a specificity of 94%, and comparison of field sample results to those of restriction fragment length polymorphism (RFLP) typing resulted in an overall consistency of >90%, except for pfdhfr51, for which most discrepancies were due to false determinations by RFLP of mixed infections. The system is sufficiently sensitive to assay parasites in clinical malaria cases and in most asymptomatic cases, and it allows high throughput with minimal hands-on time. The cost for the assay has been calculated as 0.27 euros/SNP (US$0.33), which is below the cost incurred with other systems. Due to the simplicity of the approach, newly identified SNPs can be incorporated rapidly. Such a monitoring system also makes it possible to identify the reemergence of drug-susceptible parasites once a drug has been withdrawn. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Crameri, Andreas
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
United States, Mountain View
Amunix, Inc.
Marfurt, Jutta
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Mugittu, Kefas N.
Tanzania, Ifakara
Ifakara Health Institute
Maire, Nicolas
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Regös, Attila
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Australia, Melbourne
University of Melbourne
Coppeé, Jean Yves
France, Paris
Institut Pasteur, Paris
Sismeiro, Odile
France, Paris
Institut Pasteur, Paris
Burki, Richard
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Switzerland, Bern
University of Bern
Huber, Eric
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Switzerland, Basel
F. Hoffmann-la Roche ag
Laubscher, Daniel
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Switzerland, Basel
F. Hoffmann-la Roche ag
Puijalon, Odile Mercereau
France, Paris
Institut Pasteur, Paris
Genton, Blaise
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Tanzania, Ifakara
Ifakara Health Institute
Felger, Ingrid
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Beck, Hans Peter
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Statistics
Citations: 57
Authors: 14
Affiliations: 7
Identifiers
Doi:
10.1128/JCM.01178-07
ISSN:
00951137
Research Areas
Infectious Diseases