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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
One of two different ADP-glucose pyrophosphorylase genes from potato responds strongly to elevated levels of sucrose
MGG Molecular & General Genetics, Volume 224, No. 1, Year 1990
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Description
The key regulatory step in starch biosynthesis is catalyzed by the tetrameric enzyme ADP-glucose pyrophosphorylase (AGPase). In leaf and storage tissue, the enzyme catalyzes the synthesis of ADP-glucose from glucose-l-phosphate and ATP. Using heterologous probes from maize, two sets (B and S) of cDNA clones encoding potato AGPase were isolated from a tuber-specific cDNA library. Sequence analysis revealed homology to other plant and bacterial sequences. Transcript sizes are 1.9 kb (AGPase B) and 2.1 kb (AGPase S). Northern blot experiments show that the two genes differ in their expression patterns in different organs. Furthermore, one of the genes (AGPase S) is strongly inducible by metabolizable carbohydrates (e.g. sucrose) at the RNA level. The accumulation of AGPase S mRNA was always found to be accompanied by an increase in starch content. This suggests a link between aGPase S expression and the status of a tissue as either a sink for or a source of carbohydrates. By contrast, expression of AGPase B is much less variable under various experimental conditions. © 1990 Springer-Verlag.
Authors & Co-Authors
Mueller-Roeber, Bernd
Germany, Berlin
Institut Für Genbiologische Forschung Berlin Gmbh
Kossmann, J. M.
Germany, Berlin
Institut Für Genbiologische Forschung Berlin Gmbh
Willmitzer, Lothar
Germany, Berlin
Institut Für Genbiologische Forschung Berlin Gmbh
Sonnewald, Uwe
Germany, Berlin
Institut Für Genbiologische Forschung Berlin Gmbh
Statistics
Citations: 254
Authors: 4
Affiliations: 2
Identifiers
Doi:
10.1007/BF00259460
ISSN:
16174623
Research Areas
Genetics And Genomics