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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Modified vaccinia Ankara-expressing Ag85A, a novel tuberculosis vaccine, is safe in adolescents and children, and induces polyfunctional CD4
+
T cells
European Journal of Immunology, Volume 40, No. 1, Year 2010
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Description
Modified vaccinia Ankara-expressing Ag85A (MVA85A) is a new tuberculosis (TB) vaccine aimed at enhancing immunity induced by BCG. We investigated the safety and immunogenicity of MVA85A in healthy adolescents and children from a TB endemic region, who received BCG at birth. Twelve adolescents and 24 children were vaccinated and followed up for 12 or 6 months, respectively. Adverse events were documented and vaccine-induced immune responses assessed by IFN-γ ELISpot and intracellular cytokine staining. The vaccine was well tolerated and there were no vaccine-related serious adverse events. MVA85A induced potent and durable T-cell responses. Multiple CD4+ T-cell subsets, based on expression of IFN-γ, TNF-α, IL-2, IL-17 and GM-CSF, were induced. Polyfunctional CD4+ T cells co-expressing IFN-γ, TNF-α and IL-2 dominated the response in both age groups. A novel CD4+ cell subset co-expressing these three Th1 cytokines and IL-17 was induced in adolescents, while a novel CD4+ T-cell subset co-expressing Th1 cytokines and GM-CSF was induced in children. Ag-specific CD8+ T cells were not detected.We conclude that in adolescents and children MVA85A safely induces the type of immunity thought to be important in protection against TB. This includes induction of novel Th1-cell populations that have not been previously described in humans. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.
Authors & Co-Authors
Scriba, Thomas J.
South Africa, Cape Town
University of Cape Town
Tameris, Michele D.
South Africa, Cape Town
University of Cape Town
Mansoor, Nazma
South Africa, Cape Town
University of Cape Town
Smit, Erica
South Africa, Cape Town
University of Cape Town
van der Merwe, Linda
South Africa, Cape Town
University of Cape Town
Isaacs, Fatima
South Africa, Cape Town
University of Cape Town
Keyser, Alana T.
South Africa, Cape Town
University of Cape Town
Moyo, Sizulu
South Africa, Cape Town
University of Cape Town
Brittain, Nathaniel J.
United Kingdom, Oxford
Nuffield Department of Medicine
Lawrie, Alison M.
United Kingdom, Oxford
Nuffield Department of Medicine
Gelderbloem, Sebastian J.
South Africa, Cape Town
University of Cape Town
South Africa, Cape Town
Aeras Global tb Vaccine Foundation, South Africa
Veldsman, Ashley
South Africa, Cape Town
University of Cape Town
Hatherill, Mark
South Africa, Cape Town
University of Cape Town
Hawkridge, Anthony J.
South Africa, Cape Town
Aeras Global tb Vaccine Foundation, South Africa
Hill, Adrian V. S.
United Kingdom, Oxford
Nuffield Department of Medicine
Hussey, Greg Dudley
South Africa, Cape Town
University of Cape Town
Mahomed, Hassan
South Africa, Cape Town
University of Cape Town
McShane, Helen
United Kingdom, Oxford
Nuffield Department of Medicine
Hanekom, Willem Albert
South Africa, Cape Town
University of Cape Town
Statistics
Citations: 189
Authors: 19
Affiliations: 3
Identifiers
Doi:
10.1002/eji.200939754
e-ISSN:
15214141
Research Areas
Infectious Diseases
Maternal And Child Health