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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Evaluation of methylation status of the eNOS promoter at birth in relation to childhood bone mineral content
Calcified Tissue International, Volume 90, No. 2, Year 2012
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Description
Our previous work has shown associations between childhood adiposity and perinatal methylation status of several genes in umbilical cord tissue, including endothelial nitric oxide synthase (eNOS). There is increasing evidence that eNOS is important in bone metabolism; we therefore related the methylation status of the eNOS gene promoter in stored umbilical cord to childhood bone size and density in a group of 9-year-old children. We used Sequenom MassARRAY to assess the methylation status of two CpGs in the eNOS promoter, identified from our previous study, in stored umbilical cords of 66 children who formed part of a Southampton birth cohort and who had measurements of bone size and density at age 9 years (Lunar DPXL DXA instrument). Percentage methylation varied greatly between subjects. For one of the two CpGs, eNOS chr7:150315553 +, after taking account of age and sex, there were strong positive associations between methylation status and the child's whole-body bone area (r = 0.28, P = 0.02), bone mineral content (r = 0.34, P = 0.005), and areal bone mineral density (r = 0.34, P = 0.005) at age 9 years. These associations were independent of previously documented maternal determinants of offspring bone mass. Our findings suggest an association between methylation status at birth of a specific CpG within the eNOS promoter and bone mineral content in childhood. This supports a role for eNOS in bone growth and metabolism and implies that its contribution may at least in part occur during early skeletal development. © 2011 Springer Science+Business Media, LLC.
Authors & Co-Authors
Harvey, Nicholas C.W.
United Kingdom, Southampton
Mrc Lifecourse Epidemiology Unit
Lillycrop, Karen A.
United Kingdom, Southampton
University of Southampton
Garratt, Emma S.
United Kingdom, Southampton
University of Southampton
Sheppard, Allan
New Zealand, Auckland
Liggins Institute
New Zealand, Hamilton
Agresearch Ruakura
McLean, Cameron
New Zealand, Auckland
Liggins Institute
New Zealand, Hamilton
Agresearch Ruakura
Burdge, Graham C.
United Kingdom, Southampton
University of Southampton
Slater-Jefferies, Jo
United Kingdom, Southampton
University of Southampton
Rodford, Joanne
United Kingdom, Southampton
University of Southampton
Crozier, Sarah R.
United Kingdom, Southampton
Mrc Lifecourse Epidemiology Unit
Inskip, Hazel M.
United Kingdom, Southampton
Mrc Lifecourse Epidemiology Unit
Emerald, Bright Starling
United Arab Emirates, Al Ain
College of Medicine and Health Sciences United Arab Emirates University
Gale, Catharine R.
United Kingdom, Southampton
Mrc Lifecourse Epidemiology Unit
Hanson, Mark Adrian
United Kingdom, Southampton
University of Southampton
Gluckman, Peter David
New Zealand, Auckland
Liggins Institute
Singapore, Singapore City
A-star, Singapore Institute for Clinical Sciences
Godfrey, Keith M.
United Kingdom, Southampton
Mrc Lifecourse Epidemiology Unit
United Kingdom, Southampton
University of Southampton
Cooper, Cyrus C.
United Kingdom, Southampton
Mrc Lifecourse Epidemiology Unit
Statistics
Citations: 16
Authors: 16
Affiliations: 6
Identifiers
Doi:
10.1007/s00223-011-9554-5
ISSN:
0171967X
e-ISSN:
14320827
Research Areas
Genetics And Genomics
Maternal And Child Health
Study Design
Cohort Study