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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Protective effect of a prime-boost strategy with plasmid DNA followed by recombinant adenovirus expressing TgAMA1 as vaccines against Toxoplasma gondii infection in mice
Parasitology International, Volume 61, No. 3, Year 2012
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Description
A heterologous prime-boost strategy with priming plasmid DNA followed by recombinant virus expressing relevant antigens is known to stimulate protective immunity against intracellular parasites. In this study, we have evaluated a heterologous prime-boost strategy for immunizing mice against Toxoplasma gondii infection. Our results revealed that the prime-boost strategy using both plasmid DNA and adenoviral vector encoding TgAMA1 may stimulate both humoral and Th1/Th2 cellular immune responses specific for TgAMA1. Moreover, C57BL/6 mice immunized with the pAMA1/Ad5Null, pNull/Ad5AMA1, and pAMA1/Ad5AMA1 constructs showed survival rates of 12.5%, 37.5%, and 50%, respectively. In contrast, all the pNull/Ad5Null immunized mice died after infection with the PLK-GFP strain of T. gondii. Brain cyst burden was reduced by 23% in mice immunized with pAMA1/Ad5AMA1 compared with the pNull/Ad5AMA1 immunized mice. These results demonstrate that the heterologous DNA priming and recombinant adenovirus boost strategy may provide protective immunity against T. gondii infection. © 2012 Elsevier Ireland Ltd.
Authors & Co-Authors
Yu, Longzheng
Japan, Obihiro
Obihiro University of Agriculture and Veterinary Medicine
China, Yanji
Yanbian University
Yamagishi, Junya
Japan, Obihiro
Obihiro University of Agriculture and Veterinary Medicine
Aboge, Gabriel Oluga
Japan, Obihiro
Obihiro University of Agriculture and Veterinary Medicine
Zhang, Houshuang
Japan, Obihiro
Obihiro University of Agriculture and Veterinary Medicine
Zhang, Guohong
Japan, Obihiro
Obihiro University of Agriculture and Veterinary Medicine
Tanaka, Tetsuya
Japan, Kagoshima
Kagoshima University
Fujisaki, Kozo
Japan, Kagoshima
Kagoshima University
Nishikawa, Yoshifumi
Japan, Obihiro
Obihiro University of Agriculture and Veterinary Medicine
Xuan, Xuenan
Japan, Obihiro
Obihiro University of Agriculture and Veterinary Medicine
Statistics
Citations: 24
Authors: 9
Affiliations: 3
Identifiers
Doi:
10.1016/j.parint.2012.04.001
ISSN:
18730329
Research Areas
Genetics And Genomics