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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Sequence diversity of the factor H binding protein vaccine candidate in epidemiological relevant strains of serogroup B neisseria meningitidis
Journal of Infectious Diseases, Volume 200, No. 3, Year 2009
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Description
Background. Recombinant forms of Neisseria meningitidis human factor H binding protein (fHBP) are undergoing clinical trials in candidate vaccines against invasive meningococcal serogroup B disease. We report an extensive survey and phylogenetic analysis of the diversity of fhbp genes and predicted protein sequences in invasive clinical isolates obtained in the period 2000-2006. Methods. Nucleotide sequences of fhbp genes were obtained from 1837 invasive N. meningitidis serogroup B (MnB) strains from the United States, Europe, New Zealand, and South Africa. Multilocus sequence typing (MLST) analysis was performed on a subset of the strains. Results. Every strain contained the fhbp gene. All sequences fell into 1 of 2 subfamilies (A or B), with 60%75% amino acid identity between subfamilies and at least 83% identity within each subfamily. One fHBP sequence may have arisen via inter-subfamily recombination. Subfamily B sequences were found in 70% of the isolates, and subfamily A sequences were found in 30%. Multiple fHBP variants were detected in each of the common MLST clonal complexes. All major MLST complexes include strains in both subfamily A and subfamily B. Conclusions. The diversity of strains observed underscores the importance of studying the distribution of the vaccine antigen itself rather than relying on common epidemiological surrogates such as MLST. © 2009 by the Infectious Diseases Society of America. All rights reserved.
Authors & Co-Authors
Murphy, Ellen
United States, Pearl River
Pfizer Research Pearl River
Andrew, Lubomira
United States, Pearl River
Pfizer Research Pearl River
Lee, Kwokleung
United States, Pearl River
Pfizer Research Pearl River
Dilts, Deborah A.
United States, Pearl River
Pfizer Research Pearl River
Nuñez, Lorna
United States, Pearl River
Pfizer Research Pearl River
Fink, Pamela S.
United States, Pearl River
Pfizer Research Pearl River
Ambrose, Karita D.
United States, Pearl River
Pfizer Research Pearl River
Borrow, Ray
United Kingdom, London
Public Health England
Findlow, Jamie
United Kingdom, London
Public Health England
Taha, Muhamed Kheir
France, Paris
Institut Pasteur, Paris
Deghmane, Ala Eddine
France, Paris
Institut Pasteur, Paris
Kříž, Paula
Czech Republic, Prague
National Institute of Public Health Prague
Musílek, Martin
Czech Republic, Prague
National Institute of Public Health Prague
Kalmusová, Jitka
Czech Republic, Prague
National Institute of Public Health Prague
Caugant, Dominique A.
Norway, Oslo
Folkehelseinstituttet
Alvestad, Torill
Norway, Oslo
Folkehelseinstituttet
Mayer, Leonard W.
United States, Pearl River
Pfizer Research Pearl River
United States, Atlanta
Centers for Disease Control and Prevention
Sacchi, Cláudio Tavares
United States, Pearl River
Pfizer Research Pearl River
United States, Atlanta
Centers for Disease Control and Prevention
Brazil, Cerqueira Cesar
Instituto Adolfo Lutz
Wang, Xin
United States, Pearl River
Pfizer Research Pearl River
United States, Atlanta
Centers for Disease Control and Prevention
Martín, Diana R.
New Zealand, Porirua
Esr - Environmental Science and Research
von Gottberg, Anne M.
South Africa, Johannesburg
National Institute for Communicable Diseases
Plessis, Mignon Du
South Africa, Johannesburg
National Institute for Communicable Diseases
Klugman, K. P.
United States, Atlanta
Rollins School of Public Health
South Africa, Johannesburg
National Institute for Communicable Diseases
Anderson, Annaliesa S.
United States, Pearl River
Pfizer Research Pearl River
Jansen, Kathrin Ute
United States, Pearl River
Pfizer Research Pearl River
Zlotnick, Gary W.
United States, Pearl River
Pfizer Research Pearl River
Hoiseth, Susan K.
United States, Pearl River
Pfizer Research Pearl River
Statistics
Citations: 203
Authors: 27
Affiliations: 10
Identifiers
Doi:
10.1086/600141
ISSN:
00221899
Research Areas
Genetics And Genomics
Study Design
Cross Sectional Study
Study Approach
Quantitative
Study Locations
South Africa