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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Dynamic proteomics in individual human cells uncovers widespread cell-cycle dependence of nuclear proteins
Nature Methods, Volume 3, No. 7, Year 2006
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Description
We examined cell cycle-dependent changes in the proteome of human cells by systematically measuring protein dynamics in individual living cells. We used time-lapse microscopy to measure the dynamics of a random subset of 20 nuclear proteins, each tagged with yellow fluorescent protein (YFP) at its endogenous chromosomal location. We synchronized the cells in silico by aligning protein dynamics in each cell between consecutive divisions. We observed widespread (40%) cell-cycle dependence of nuclear protein levels and detected previously unknown cell cycle-dependent localization changes. This approach to dynamic proteomics can aid in discovery and accurate quantification of the extensive regulation of protein concentration and localization in individual living cells. © 2006 Nature Publishing Group.
Authors & Co-Authors
Sigal, Alex
Israel, Rehovot
Weizmann Institute of Science Israel
Milo, Ron
Israel, Rehovot
Weizmann Institute of Science Israel
Carpenter, Anne Elizabeth
United States, Cambridge
Whitehead Institute
Statistics
Citations: 112
Authors: 3
Affiliations: 4
Identifiers
Doi:
10.1038/nmeth892
ISSN:
15491676
Research Areas
Cancer