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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Effect of antioxidant supplementation on the sperm proteome of idiopathic infertile men
Antioxidants, Volume 8, No. 10, Article 488, Year 2019
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Description
Antioxidant supplementation in idiopathic male infertility has a beneficial effect on semen parameters. However, the molecular mechanism behind this effect has not been reported. The objective of this study was to evaluate the sperm proteome of idiopathic infertile men pre-and post-antioxidant supplementation. Idiopathic infertile men were provided with oral antioxidant supplementation once daily for a period of 6 months. Of the 379 differentially expressed proteins (DEPs) between pre-and post-antioxidant treatment patients, the majority of the proteins (n = 274) were overexpressed following antioxidant treatment. Bioinformatic analysis revealed the activation of oxidative phosphorylation pathway and upregulation of key proteins involved in spermatogenesis, sperm maturation, binding of sperm, fertilization and normal reproductive function. In addition, the transcriptional factors associated with antioxidant defense system (PPARGC1A) and free radical scavenging (NFE2L2) were predicted to be functionally activated post-treatment. Key DEPs, namely, NDUFS1, CCT3, PRKARA1 and SPA17 validated by Western blot showed significant overexpression post-treatment. Our novel proteomic findings suggest that antioxidant supplementation in idiopathic infertile men improves sperm function at the molecular level by modulating proteins involved in CREM signaling, mitochondrial function and protein oxidation. Further, activation of TRiC complex helped in nuclear compaction, maintenance of telomere length, flagella function, and expression of zona pellucida receptors for sperm–oocyte interaction. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC6827009/bin/antioxidants-08-00488-s001.zip
Authors & Co-Authors
Agarwal, Ashok K.
United States, Cleveland
Cleveland Clinic Foundation
Panner Selvam, Manesh Kumar
United States, Cleveland
Cleveland Clinic Foundation
Samanta, Luna
United States, Cleveland
Cleveland Clinic Foundation
India, Cuttack
Ravenshaw University
Vij, Sarah Coleman
United States, Cleveland
Cleveland Clinic Foundation
Parekh, Neel V.
United States, Cleveland
Cleveland Clinic Foundation
Sabanegh, Edmund
United States, Cleveland
Cleveland Clinic Foundation
Tadros, Nicholas N.
United States, Springfield
Siu School of Medicine
Arafa, Mohamed Mostafa
Qatar, Doha
Hamad Medical Corporation
Sharma, Rakesh Kumar
United States, Cleveland
Cleveland Clinic Foundation
Statistics
Citations: 21
Authors: 9
Affiliations: 4
Identifiers
Doi:
10.3390/antiox8100488
ISSN:
20763921
Research Areas
Cancer
Health System And Policy
Sexual And Reproductive Health
Participants Gender
Male