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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Atomic force microscopy study of the effect of antimicrobial peptides on the cell envelope of Escherichia coli
Antimicrobial Agents and Chemotherapy, Volume 49, No. 10, Year 2005
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Description
The influences of the antibacterial magainin 2 and PGLa from the African clawed frog (Xenopus laevis) and the hemolytic bee venom melittin on Escherichia coli as the target cell were studied by atomic force microscopy (AFM). Nanometer-scale images of the effects of the peptides on this gram-negative bacterium's cell envelope were obtained in situ without the use of fixing agents. These high-resolution AFM images of the surviving and intact target cells before and after peptide treatment showed distinct changes in cell envelope morphology as a consequence of peptide action. Although all three peptides are lytic to E. coli, it is clear from this AFM study that each peptide causes distinct morphological changes in the outer membrane and in some cases the inner membrane, probably as a consequence of different mechanisms of action. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Meincken, Martina
South Africa, Stellenbosch
Stellenbosch University
Holroyd, D. L.
South Africa, Stellenbosch
Stellenbosch University
Rautenbach, Marina
South Africa, Stellenbosch
Stellenbosch University
Statistics
Citations: 178
Authors: 3
Affiliations: 1
Identifiers
Doi:
10.1128/AAC.49.10.4085-4092.2005
ISSN:
00664804