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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
A world-wide analysis of reduced sensitivity to DMI fungicides in the banana pathogen Pseudocercospora fijiensis
Pest Management Science, Volume 77, No. 7, Year 2021
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Description
BACKGROUND: Pseudocercospora fijiensis is the causal agent of the black leaf streak disease (BLSD) of banana. Bananas are important global export commodities and a major staple food. Their susceptibility to BLSD pushes disease management towards excessive fungicide use, largely relying on multisite inhibitors and sterol demethylation inhibitors (DMIs). These fungicides are ubiquitous in plant disease control, targeting the CYP51 enzyme. We examined sensitivity to DMIs in P. fijiensis field isolates collected from various major banana production zones in Colombia, Costa Rica, Dominican Republic, Ecuador, the Philippines, Guadalupe, Martinique and Cameroon and determined the underlying genetic reasons for the observed phenotypes. RESULTS: We observed a continuous range of sensitivity towards the DMI fungicides difenoconazole, epoxiconazole and propiconazole with clear cross-sensitivity. Sequence analyses of PfCYP51 in 266 isolates showed 28 independent amino acid substitutions, nine of which correlated with reduced sensitivity to DMIs. In addition to the mutations, we observed up to six insertions in the Pfcyp51 promoter. Such promoter insertions contain repeated elements with a palindromic core and correlate with the enhanced expression of Pfcyp51 and hence with reduced DMI sensitivity. Wild-type isolates from unsprayed bananas fields did not contain any promoter insertions. CONCLUSION: The presented data significantly contribute to understanding of the evolution and global distribution of DMI resistance mechanisms in P. fijiensis field populations and facilitate the prediction of different DMI efficacy. The overall reduced DMI sensitivity calls for the deployment of a wider range of solutions for sustainable control of this major banana disease. © 2021 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC8252799/bin/PS-77-3273-s008.xlsx
https://efashare.b-cdn.net/share/pmc/articles/PMC8252799/bin/PS-77-3273-s002.docx
https://efashare.b-cdn.net/share/pmc/articles/PMC8252799/bin/PS-77-3273-s001.xlsx
https://efashare.b-cdn.net/share/pmc/articles/PMC8252799/bin/PS-77-3273-s005.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC8252799/bin/PS-77-3273-s006.docx
https://efashare.b-cdn.net/share/pmc/articles/PMC8252799/bin/PS-77-3273-s012.pdf
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https://efashare.b-cdn.net/share/pmc/articles/PMC8252799/bin/PS-77-3273-s009.docx
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https://efashare.b-cdn.net/share/pmc/articles/PMC8252799/bin/PS-77-3273-s010.docx
Authors & Co-Authors
Chong, Pablo
Unknown Affiliation
Essoh, Josué Ngando
Unknown Affiliation
Arango Isaza, Rafael E.
Unknown Affiliation
Keizer, Paul
Unknown Affiliation
Stergiopoulos, Ioannis
Unknown Affiliation
Seidl, Michael F.
Unknown Affiliation
Guzmán, Mauricio
Unknown Affiliation
Sandoval, Jorge A.
Unknown Affiliation
Verweij, Paul E.
Unknown Affiliation
Scalliet, Gabriel
Unknown Affiliation
Sierotzski, Helge
Unknown Affiliation
De Lapeyre de Bellaire, Luc
Unknown Affiliation
Crous, Pedro Willem
Unknown Affiliation
Carlier, Jean
Unknown Affiliation
Cros, Sandrine
Unknown Affiliation
Meijer, Harold J.G.
Unknown Affiliation
Peralta, Esther Lilia
Unknown Affiliation
Kema, Gerrit H.J.
Unknown Affiliation
Statistics
Citations: 18
Authors: 18
Affiliations: 15
Identifiers
Doi:
10.1002/ps.6372
ISSN:
1526498X
e-ISSN:
15264998
Research Areas
Food Security
Genetics And Genomics
Study Locations
Cameroon