High-level resistance to colistin mediated by various mutations in the crrB gene among carbapenemase-producing klebsiella pneumoniae
Antimicrobial Agents and Chemotherapy, Volume 61, No. 11, Article e01423-17, Year 2017
Notification
URL copied to clipboard!
Mutations in crrAB genes encoding a two-component regulator involved in modifications of lipopolysaccharide were searched for among a collection of colistin-resistant Klebsiella pneumoniae isolates. Four isolates, respectively, producing carbapen-emases NDM-1, OXA-181, or KPC-2 showed mutated CrrB proteins compared with those in wild-type strains. Complementation assays with a wild-type CrrB protein restored the susceptibility to colistin in all cases, confirming the involvement of the identified substitutions in the resistance phenotype.