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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Promiscuous expression of Epstein-Barr virus genes in Burkitt's lymphoma from the central African country Malawi
International Journal of Cancer, Volume 99, No. 5, Year 2002
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Description
Primary BL in Malawian children has a very high frequency association, approaching 100%, with the human herpesvirus EBV. A detailed study carried out on viral gene expression in these tumours, using both fresh material and methanol-fixed FNAs, showed, contrary to prediction, that most belong to a variant "class II" latency category, with lytic cycle-related genes also expressed. That is, in addition to EBNA I expression, membrane proteins (LMPI/2A), immediate early (BZLFI) and early (IR2 and IR4) genes, a putative viral oncogene (BARFI), CST (BART) antisense transcripts and the viral bcl-2 homologue are expressed in a high proportion of the BLs. Most, but not all, express the small viral (EBER) RNAs. Two other significant observations were made: (i) in addition to expression of cellular cytokine (IL-10) transcripts in all tumours investigated, the normally silent viral IL-10 homologue was expressed in some tumours; (ii) whereas EBNA I expression from its restricted Qp promoter was generally observed, the nonrestricted Cp/Wp promoter was also active in some tumours. Viral gene expression in the Malawian [endemic (e)] BLs appears to be more promiscuous than predicted from other studies, but expression accords with the cytopathologic picture of eBLs as a rapidly proliferating cell population accompanied by considerable necrosis, and a clinically diverse disease. A small-scale study of relapse Malawian BLs revealed a different picture of viral association, more akin to systemic BL than eBL, where EBV appears to be absent or present only at very low levels. The significance of these findings is considered. © 2002 Wiley-Liss, Inc.
Authors & Co-Authors
Xue, Shaoan
United Kingdom, London
Imperial College London
China, Xi'an
Shaanxi Normal University
Labrecque, Louise Geneviève
Canada, Montreal
Centre Hospitalier de L'universite de Montreal
Lu, Qi Long
United Kingdom, London
Hammersmith Hospital
Ong, S. Kate
United Kingdom, London
Imperial College London
Lampert, Irvin A.
United Kingdom, London
Hammersmith Hospital
Kazembe, Peter Nicholas
Malawi, Lilongwe
Lilongwe Central Hospital
Molyneux, Elizabeth M.
Malawi, Zomba
University of Malawi
Broadhead, Robin L.
Malawi, Zomba
University of Malawi
Borgstein, Eric S.
Malawi, Zomba
University of Malawi
Griffin, Beverly E.
United Kingdom, London
Imperial College London
Statistics
Citations: 115
Authors: 10
Affiliations: 6
Identifiers
Doi:
10.1002/ijc.10372
ISSN:
00207136
Research Areas
Genetics And Genomics
Maternal And Child Health
Study Design
Cross Sectional Study
Study Locations
Malawi