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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Serological evaluation of mycobacterium ulcerans Antigens identified by comparative genomics
PLoS Neglected Tropical Diseases, Volume 4, No. 11, Article e872, Year 2010
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Description
A specific and sensitive serodiagnostic test for Mycobacterium ulcerans infection would greatly assist the diagnosis of Buruli ulcer and would also facilitate seroepidemiological surveys. By comparative genomics, we identified 45 potential M. ulcerans specific proteins, of which we were able to express and purify 33 in E. coli. Sera from 30 confirmed Buruli ulcer patients, 24 healthy controls from the same endemic region and 30 healthy controls from a non-endemic region in Benin were screened for antibody responses to these specific proteins by ELISA. Serum IgG responses of Buruli ulcer patients were highly variable, however, seven proteins (MUP045, MUP057, MUL_0513, Hsp65, and the polyketide synthase domains ER, AT propionate, and KR A) showed a significant difference between patient and non-endemic control antibody responses. However, when sera from the healthy control subjects living in the same Buruli ulcer endemic area as the patients were examined, none of the proteins were able to discriminate between these two groups. Nevertheless, six of the seven proteins showed an ability to distinguish people living in an endemic area from those in a non-endemic area with an average sensitivity of 69% and specificity of 88%, suggesting exposure to M. ulcerans. Further validation of these six proteins is now underway to assess their suitability for use in Buruli ulcer seroepidemiological studies. Such studies are urgently needed to assist efforts to uncover environmental reservoirs and understand transmission pathways of the M. ulcerans. © 2010 Pidot et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC2970529/bin/pntd.0000872.s001.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC2970529/bin/pntd.0000872.s002.doc
https://efashare.b-cdn.net/share/pmc/articles/PMC2970529/bin/pntd.0000872.s003.doc
https://efashare.b-cdn.net/share/pmc/articles/PMC2970529/bin/pntd.0000872.s004.doc
Authors & Co-Authors
Pidot, Sacha J.
Australia, Clayton
Monash University
Australia, Melbourne
University of Melbourne
Porter, Jessica L.
Australia, Melbourne
University of Melbourne
Marsollier, Laurent
France, Angers
Université D’angers
Chauty, Annick
Benin
Centre de Dépistage et de Traitement de L'ulcère de Buruli
Migot-Nabias, Florence
France, Marseille
Ird Institut de Recherche Pour le Developpement
France, Paris
Université Paris Cité
Badaut, Cyril
France, Marseille
Ird Institut de Recherche Pour le Developpement
France, Paris
Université Paris Cité
Bénard, Angéle Bénard
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Ruf, Marie Thérèse
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Seemann, Torsten
Australia, Clayton
Monash University
Johnson, Paul D.R.
Australia, Melbourne
Austin Health
Davies, John K.
Australia, Clayton
Monash University
Jenkin, Grant A.
Australia, Clayton
Monash University
Australia, Melbourne
University of Melbourne
Pluschke, Gerd
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Stinear, Timothy Paul
Australia, Clayton
Monash University
Australia, Melbourne
University of Melbourne
Statistics
Citations: 43
Authors: 14
Affiliations: 8
Identifiers
Doi:
10.1371/journal.pntd.0000872
Research Areas
Cancer
Genetics And Genomics
Health System And Policy
Study Design
Cross Sectional Study
Study Locations
Benin