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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
general
Drying a tuberculosis vaccine without freezing
Proceedings of the National Academy of Sciences of the United States of America, Volume 104, No. 8, Year 2007
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Description
With the increasing incidence of tuberculosis and drug resistant disease in developing countries due to HIV/AIDS, there is a need for vaccines that are more effective than the present bacillus Calmette-Guérin (BCG) vaccine. We demonstrate that BCG vaccine can be dried without traditional freezing and maintained with remarkable refrigerated and room-temperature stability for months through spray drying. Studies with a model Mycobacterium (Mycobacterium smegmatis) revealed that by removing salts and cryoprotectant (e.g., glycerol) from bacterial suspensions, the significant osmotic pressures that are normally produced on bacterial membranes through droplet drying can be reduced sufficiently to minimize loss of viability on drying by up to 2 orders of magnitude. By placing the bacteria in a matrix of leucine, high-yield, free-flowing, "vial-fillable" powders of bacteria (including M. smegmatis and M. bovis BCG) can be produced. These powders show relatively minor losses of activity after maintenance at 4°C and 25°C up to and beyond 4 months. Comparisons with lyophilized material prepared both with the same formulation and with a commercial formulation reveal that the spray-dried BCG has better overall viability on drying. © 2007 by The National Academy of Sciences of the USA.
Authors & Co-Authors
Wong, Yunling
United States, Cambridge
Harvard University
Sampson, Samantha Leigh
United States, Boston
Harvard T.h. Chan School of Public Health
Germishuizen, Willem Andreas
United States, Cambridge
Harvard University
Goonesekera, Sunali D.
United States, Boston
Harvard T.h. Chan School of Public Health
Sadoff, Jerald C.
United States, Rockville
Aeras Global tb Vaccine Foundation
Bloom, Barry R.
United States, Boston
Harvard T.h. Chan School of Public Health
Edwards, David A.
United States, Cambridge
Harvard University
Statistics
Citations: 80
Authors: 7
Affiliations: 3
Identifiers
Doi:
10.1073/pnas.0611430104
ISSN:
00278424
Research Areas
Infectious Diseases
Study Design
Cohort Study