Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Artificial rearing of infant mice leads to n-3 fatty acid deficiency in cardiac, neural and peripheral tissues
Lipids, Volume 44, No. 8, Year 2009
Notification
URL copied to clipboard!
Description
The ability to control the fatty acid content of the diet during early development is a crucial requirement for a one-generation model of docosahexaenoic acid (DHA; 22:6n3) deficiency. A hand feeding method using artificial rearing (AR) together with sterile, artificial milk was employed for feeding mice from postnatal day 2-15. The pups were fed an n-3 fatty acid adequate (3% α-linolenic acid (LNA; 18:3n3) + 1% 22:6n3) or a deficient diet (0.06% 18:3n3) with linoleic acid (LA; 18:2n6) as the only dietary source of essential fatty acids by AR along with a dam-reared control group (3.1% 18:3n3). The results indicate that restriction of n-3 fatty acid intake during postnatal development leads to markedly lower levels of brain, retinal, liver, plasma and heart 22:6n3 at 20 weeks of age with replacement by docosapentaenoic acid (DPAn6; 22:5n6), arachidonic acid (ARA; 20:4n6) and docosatetraenoic acid (DTA; 22:4n6). A detailed analysis of phospholipid classes of heart tissue indicated that phosphatidylethanolamine, phosphatidylcholine and cardiolipin were the major repositories of 22:6n3, reaching 40, 29 and 15%, respectively. A novel heart cardiolipin species containing four 22:6n3 moieties is described. This is the first report of the application of artificially rearing to mouse pup nutrition; this technique will facilitate dietary studies of knockout animals as well as the study of essential fatty acid (EFA) functions in the cardiovascular, neural and other organ systems. © 2009 US Government.
Authors & Co-Authors
Hussein, Nahed
Egypt, Cairo
Faculty of Specific Education Ain Shams University
Fedorova, Irina
United States, Bethesda
National Institute on Alcohol Abuse and Alcoholism Niaaa
Moriguchi, Toru
United States, Bethesda
National Institute on Alcohol Abuse and Alcoholism Niaaa
Hamazaki, Kei
United States, Bethesda
National Institute on Alcohol Abuse and Alcoholism Niaaa
Kim, Hee Yong
United States, Bethesda
National Institute on Alcohol Abuse and Alcoholism Niaaa
Hoshiba, Junji
Japan, Okayama
Okayama University
Salem, Norman
United States, Columbia
Martek Biosciences Corporation
Statistics
Citations: 22
Authors: 7
Affiliations: 4
Identifiers
Doi:
10.1007/s11745-009-3318-2
ISSN:
00244201
e-ISSN:
15589307
Research Areas
Food Security
Health System And Policy
Maternal And Child Health
Noncommunicable Diseases
Study Design
Randomised Control Trial