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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Cytokines secreted by macrophages isolated from tumor microenvironment of inflammatory breast cancer patients possess chemotactic properties
International Journal of Biochemistry and Cell Biology, Volume 46, No. 1, Year 2014
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Description
Although there is a growing literature describing the role of macrophages in breast cancer, the role of macrophages in inflammatory breast cancer (IBC) is unclear. The aim of present study was to isolate and characterize tumor associated macrophages of IBC and non-IBC patients and define their role in IBC. Tumor infiltrating monocytes/macrophages (CD14+ and CD68+) were measured by immunohistochemistry using specific monoclonal antibodies. Blood drained from axillary vein tributaries was collected during breast cancer surgery and the percentage of CD14+ in the total isolated leukocytes was assessed by flow cytometric analysis. CD14+ cells were separated from total leukocytes by immuno-magnetic beads technique and were cultured overnight. Media conditioned by CD14+ were collected and subjected to cytokine profiling using cytokine antibody array. Wound healing and invasion assays were used to test whether cytokines highly secreted by tumor drained macrophages induce motility and invasion of breast cancer cells. We found that macrophages highly infiltrate into carcinoma tissues of IBC patients. In addition blood collected from axillary tributaries of IBC patients is highly enriched with CD14+ cells as compared to blood collected from non-IBC patients. Cytokine profiling of CD14+ cells isolated from IBC patients revealed a significant increase in secretion of tumor necrosis factor-α; monocyte chemoattractant protein-1/CC-chemokine ligand 2; interleukin-8 and interleukin-10 as compared to CD14+ cells isolated from non-IBC patients. Tumor necrosis factor-α, interleukin-8 and interleukin-10 significantly increased motility and invasion of IBC cells in vitro. In conclusion, macrophages isolated from the tumor microenvironment of IBC patients secrete chemotactic cytokines that may augment dissemination and metastasis of IBC carcinoma cells. © 2013 Elsevier Ltd.
Authors & Co-Authors
Mohamed, Mona M.
Egypt, Giza
Faculty of Science
El-Ghonaimy, Eslam A.
Egypt, Giza
Faculty of Science
Nouh, M. Akram
Egypt, Giza
National Cancer Institute
Schneider, Robert J.
United States, New York
New York University
Sloane, Bonnie F.
United States, Detroit
Wayne State University School of Medicine
El-Shinawi, Mohamed
Egypt, Cairo
Faculty of Medicine - Ain Shams University
Statistics
Citations: 85
Authors: 6
Affiliations: 5
Identifiers
Doi:
10.1016/j.biocel.2013.11.015
ISSN:
13572725
e-ISSN:
18785875
Research Areas
Cancer
Health System And Policy