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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Ivermectin selection on β-tubulin: Evidence in Onchocerca volvulus and Haemonchus contortus
Molecular and Biochemical Parasitology, Volume 150, No. 2, Year 2006
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Description
Ivermectin resistance is common in trichostrongylid nematodes of livestock, such as Haemonchus contortus. This anthelmintic is the only drug approved for mass administration to control onchocerciasis caused by the nematode parasite, Onchocerca volvulus. In parts of West Africa up to 18 rounds of ivermectin treatment have been administered to communities and there are reports of poor parasitological responses to treatment. Understanding ivermectin resistance and ivermectin selection is an important step to reduce selection pressure for resistance, and to develop molecular markers which can be used to monitor the development of resistance and its spread. Here we report evidence that ivermectin selection changes the frequency of β-tubulin alleles in both the sheep parasite, H. contortus, and the human parasite, O. volvulus. In O. volvulus we have been able to look at the frequency of β-tubulin alleles in O. volvulus obtained before any ivermectin was used in humans in Africa, and following its widespread use. In H. contortus, we have been able to look at the frequency of β-tubulin alleles in a strain which has not seen any anthelmintic selection and in an ivermectin selected strain derived from the unselected strain. We have found ivermectin selects on β-tubulin in both of these nematode species. In the case of O. volvulus, we had previously reported that ivermectin selects for specific single nucleotide polymorphisms in the O. volvulus β-tubulin gene. This polymorphism results in three amino acid changes in the H3 helix of β-tubulin, as well as deletions in an associated intron. We report a simple PCR assay to detect the amplicon length polymorphism, resulting from these intronic deletions, which can be used to monitor the frequency of the β-tubulin allele selected for by ivermectin in O. volvulus. © 2006 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Eng, Jeffrey KL
Canada, Sainte-anne-de-bellevue
Mcgill University, Macdonald Campus
Blackhall, W. J.
Germany, Hannover
Tierärztliche Hochschule Hannover
Osei-Atweneboana, Mike Yaw
Canada, Sainte-anne-de-bellevue
Mcgill University, Macdonald Campus
Bourguinat, Catherine
Canada, Sainte-anne-de-bellevue
Mcgill University, Macdonald Campus
Galazzo, D.
Canada, Sainte-anne-de-bellevue
Mcgill University, Macdonald Campus
Beech, R. N.
Canada, Sainte-anne-de-bellevue
Mcgill University, Macdonald Campus
Unnasch, Thomas Raymond
United States, Birmingham
The University of Alabama at Birmingham
Awadzi, Kwablah
Ghana
Onchocerciasis Research Centre
Lubega, George William
Uganda, Kampala
Makerere University
Prichard, Roger K.
Canada, Sainte-anne-de-bellevue
Mcgill University, Macdonald Campus
Statistics
Citations: 99
Authors: 10
Affiliations: 5
Identifiers
Doi:
10.1016/j.molbiopara.2006.08.007
ISSN:
01666851
Research Areas
Genetics And Genomics
Infectious Diseases
Study Locations
Multi-countries