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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
veterinary
Generation of recombinant antibody fragments that target canine dendritic cells by phage display technology
Veterinary and Comparative Oncology, Volume 9, No. 3, Year 2011
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Description
One of the main goals in cancer immunotherapy is the efficient activation of the host immune system against tumour cells. Dendritic cells (DCs) can induce specific anti-tumour immune responses in both experimental animal models and humans. However, most preclinical studies using small animal models show only limited correlation with studies carried out in clinical settings, whereas laboratory dogs naturally develop tumours that are biologically and histopathologically similar to their human counterparts. Here, we describe the generation and characterization of recombinant antibodies against canine DCs, isolated using the Tomlinson phage display system. We successfully isolated highly specific single-chain variable fragment (scFv) antibodies in a sequential three-step panning strategy involving depletion on canine peripheral blood mononuclear cells followed by positive selection on native canine DCs. This provides the basis for an antibody-based method for the immunological detection and manipulation of DCs and for monitoring antigen-specific immune responses. © 2010 Blackwell Publishing Ltd.
Authors & Co-Authors
Junghanss, Christian
Germany, Rostock
Universität Rostock
Willenbrock, Saskia
Germany, Hannover
Tierärztliche Hochschule Hannover
Murua-Escobar, Hugo
Germany, Hannover
Tierärztliche Hochschule Hannover
Nolte, Ingo J.A.
Germany, Hannover
Tierärztliche Hochschule Hannover
Barth, Stefan W.
Germany, Aachen
Rheinisch-westfälische Technische Hochschule Aachen
Germany, Aachen
Fraunhofer Institute for Molecular Biology and Applied Ecology Ime
Tur, Mehmet Kemal
Germany, Aachen
Rheinisch-westfälische Technische Hochschule Aachen
Statistics
Citations: 10
Authors: 6
Affiliations: 4
Identifiers
Doi:
10.1111/j.1476-5829.2010.00246.x
ISSN:
14765829
Research Areas
Cancer