Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Multiple mycotoxin exposure determined by urinary biomarkers in rural subsistence farmers in the former Transkei, South Africa
Food and Chemical Toxicology, Volume 62, Year 2013
Notification
URL copied to clipboard!
Description
Subsistence farmers are exposed to a range of mycotoxins. This study applied novel urinary multi-mycotoxin LC-MS/MS methods to determine multiple exposure biomarkers in the high oesophageal cancer region, Transkei, South Africa. Fifty-three female participants donated part of their maize-based evening meal and first void morning urine, which was analysed both with sample clean-up (single and multi-biomarker) and by a 'dilute-and-shoot' multi-biomarker method. Results were corrected for recovery with LOD for not detected. A single biomarker method detected fumonisin B1 (FB1) (87% incidence; mean±standard deviation 0.342±0.466ng/mg creatinine) and deoxynivalenol (100%; mean 20.4±49.4ng/mg creatinine) after hydrolysis with β-glucuronidase. The multi-biomarker 'dilute-and-shoot' method indicated deoxynivalenol-15-glucuronide was predominantly present. A multi-biomarker method with β-glucuronidase and immunoaffinity clean-up determined zearalenone (100%; 0.529±1.60ng/mg creatinine), FB1 (96%; 1.52±2.17ng/mg creatinine), α-zearalenol (92%; 0.614±1.91ng/mg creatinine), deoxynivalenol (87%; 11.3±27.1ng/mg creatinine), β-zearalenol (75%; 0.702±2.95ng/mg creatinine) and ochratoxin A (98%; 0.041±0.086ng/mg creatinine). These demonstrate the value of multi-biomarker methods in measuring exposures in populations exposed to multiple mycotoxins. This is the first finding of urinary deoxynivalenol, zearalenone, their conjugates, ochratoxin A and zearalenols in Transkei. © 2013 Elsevier Ltd.
Authors & Co-Authors
Shephard, Gordon Seymour
South Africa, Tygerberg
South African Medical Research Council
Burger, Hester Mari
South Africa, Tygerberg
South African Medical Research Council
Gambacorta, Lucia
Italy, Bari
Istituto Di Scienze Delle Produzioni Alimentari
Gong, Yun Yun
United Kingdom, Leeds
University of Leeds, School of Medicine
United Kingdom, Belfast
Queen's University Belfast
Krska, Rudolf
Austria, Tulln
Center for Analytical Chemistry, Tulln
Rheeder, John P.
South Africa, Tygerberg
South African Medical Research Council
Solfrizzo, M.
Italy, Bari
Istituto Di Scienze Delle Produzioni Alimentari
Srey, Chou
United Kingdom, Leeds
University of Leeds, School of Medicine
United Kingdom, Belfast
Queen's University Belfast
Sulyok, Michael
Austria, Tulln
Center for Analytical Chemistry, Tulln
Visconti, Angelo
Italy, Bari
Istituto Di Scienze Delle Produzioni Alimentari
Warth, Benedikt
Austria, Tulln
Center for Analytical Chemistry, Tulln
van der Westhuizen, Liana
South Africa, Tygerberg
South African Medical Research Council
Statistics
Citations: 136
Authors: 12
Affiliations: 5
Identifiers
Doi:
10.1016/j.fct.2013.08.040
ISSN:
02786915
e-ISSN:
18736351
Research Areas
Cancer
Study Design
Cohort Study
Study Locations
South Africa
Participants Gender
Female