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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Differential responses to natural and recombinant allergens in a murine model of fish allergy
Molecular Immunology, Volume 48, No. 4, Year 2011
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Description
Aerosolized fish proteins are an important cause of allergic airway reactions in both the domestic and the occupational environment. The aim of this study was to investigate inhalant fish-induced allergy in a mouse model and compare immune responses generated by raw and heat-treated fish extracts as well as natural and recombinant forms of the major fish allergen parvalbumin. Mice were sensitized with raw or cooked pilchard extract and challenged intranasally with cooked pilchard extract, purified natural pilchard parvalbumin or recombinant carp parvalbumin (rCyp c1.01). Cooked pilchard extract predominantly sensitized mice to parvalbumin and induced specific IgG1 and IgE antibodies against both pilchard parvalbumin and rCyp c1.01, whereas additional allergens were recognized by mice sensitized with raw extract, including a 36 kDa allergen that was also recognized by fish processing workers and was identified as glyceraldehyde-3-phosphate dehydrogenase. Mice challenged with cooked extract and purified pilchard parvalbumin had increased Th2 cytokine production in mediastinal lymph node cells and splenocytes, whereas mice challenged with rCyp c1.01 did not. This study identifies a new IgE-binding protein that may be important in occupational allergy to fish and demonstrates the feasibility of testing recombinant allergens for immunotherapeutic potential in vivo. © 2010 Elsevier Ltd.
Authors & Co-Authors
van der Ventel, Michelle L.
South Africa, Cape Town
Faculty of Health Sciences
Nieuwenhuizen, Natalie Eva
South Africa, Cape Town
Faculty of Health Sciences
Italy, Trieste
International Centre for Genetic Engineering and Biotechnology
Kirstein, Frank
South Africa, Cape Town
Faculty of Health Sciences
Italy, Trieste
International Centre for Genetic Engineering and Biotechnology
Hikuam, Christoph
South Africa, Cape Town
Faculty of Health Sciences
Italy, Trieste
International Centre for Genetic Engineering and Biotechnology
South Africa, Bellville
Cape Peninsula University of Technology
Jeebhay, Mohamed Fareed
South Africa, Cape Town
Faculty of Health Sciences
Swoboda, Ines
Austria, Vienna
Medizinische Universität Wien
Brombacher, Frank
South Africa, Cape Town
Faculty of Health Sciences
Italy, Trieste
International Centre for Genetic Engineering and Biotechnology
Lopata, Andreas Ludwig
South Africa, Cape Town
Faculty of Health Sciences
Statistics
Citations: 51
Authors: 8
Affiliations: 4
Identifiers
Doi:
10.1016/j.molimm.2010.11.001