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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Genomic and functional analysis of emerging virulent and multidrug-resistant Escherichia coli lineage sequence type 648
Antimicrobial Agents and Chemotherapy, Volume 63, No. 6, Article e00243-19, Year 2019
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Description
The pathogenic extended-spectrum-beta-lactamase (ESBL)-producing Escherichia coli lineage ST648 is increasingly reported from multiple origins. Our study of a large and global ST648 collection from various hosts (87 whole-genome sequences) combining core and accessory genomics with functional analyses and in vivo experiments suggests that ST648 is a nascent and generalist lineage, lacking clear phylogeographic and host association signals. By including large numbers of ST131 (n 107) and ST10 (n 96) strains for comparative genomics and phenotypic analysis, we demonstrate that the combination of multidrug resistance and high-level virulence are the hallmarks of ST648, similar to international high-risk clonal lineage ST131. Specifically, our in silico, in vitro, and in vivo results demonstrate that ST648 is well equipped with biofilm-associated features, while ST131 shows sophisticated signatures indicative of adaption to urinary tract infection, potentially conveying individual ecological niche adaptation. In addition, we used a recently developed NFDS (negative frequency-dependent selection) population model suggesting that ST648 will increase significantly in frequency as a cause of bacteremia within the next few years. Also, ESBL plasmids impacting biofilm formation aided in shaping and maintaining ST648 strains to successfully emerge worldwide across different ecologies. Our study contributes to understanding what factors drive the evolution and spread of emerging international high-risk clonal lineages. Copyright © 2019 American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Schaufler, Katharina
Germany, Greifswald
Universität Greifswald
Germany, Berlin
Freie Universität Berlin
Semmler, Torsten
Germany, Berlin
Robert Koch Institute
Wieler, Lothar H.
Germany, Berlin
Robert Koch Institute
Trott, Darren John
Australia, Adelaide
The University of Adelaide
Pitout, Johann D.D.
Canada, Calgary
Calgary Laboratory Services
Canada, Calgary
University of Calgary
Peirano, Gisele
Canada, Calgary
Calgary Laboratory Services
Canada, Calgary
University of Calgary
Bonnedahl, Jonas
Sweden, Linkoping
Linköpings Universitet
Dolejská, Monika
Czech Republic, Brno
Veterinární Univerzita Brno
Czech Republic, Brno
Středoevropský Technologický Institut
Literák, Ivan
Czech Republic, Brno
Veterinární Univerzita Brno
Czech Republic, Brno
Středoevropský Technologický Institut
Fuchs, Stephan
Germany, Berlin
Robert Koch Institute
Ahmed, Niyaz
Bangladesh, Dhaka
International Centre for Diarrhoeal Disease Research Bangladesh
Torres, Carmen
Spain, Logrono
Universidad de la Rioja
McNally, Alan
United Kingdom, Birmingham
University of Birmingham
Pickard, Derek John Juan
United Kingdom, Hinxton
Wellcome Sanger Institute
Ewers, Christa
Germany, Giessen
Justus-liebig-universität Gießen
Croucher, Nicholas J.
United Kingdom, London
Imperial College London
Corander, Jukka
United Kingdom, Hinxton
Wellcome Sanger Institute
Finland, Helsinki
Helsingin Yliopisto
Norway, Oslo
Universitetet I Oslo
Guenther, Sebastian
Germany, Berlin
Freie Universität Berlin
Germany, Greifswald
Universität Greifswald
Statistics
Citations: 56
Authors: 18
Affiliations: 18
Identifiers
Doi:
10.1128/AAC.00243-19
ISSN:
00664804
Research Areas
Cancer
Genetics And Genomics
Study Design
Cross Sectional Study