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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Functional memory B cells and long-lived plasma cells are generated after a single Plasmodium chabaudi infection in mice
PLoS Pathogens, Volume 5, No. 12, Article e1000690, Year 2009
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Description
Antibodies have long been shown to play a critical role in naturally acquired immunity to malaria, but it has been suggested that Plasmodium-specific antibodies in humans may not be long lived. The cellular mechanisms underlying B cell and antibody responses are difficult to study in human infections; therefore, we have investigated the kinetics, duration and characteristics of the Plasmodium-specific memory B cell response in an infection of P. chabaudi in mice. Memory B cells and plasma cells specific for the C-terminal region of Merozoite Surface Protein 1 were detectable for more than eight months following primary infection. Furthermore, a classical memory response comprised predominantly of the T-cell dependent isotypes IgG2c, IgG2b and IgG1 was elicited upon rechallenge with the homologous parasite, confirming the generation of functional memory B cells. Using cyclophosphamide treatment to discriminate between long-lived and short-lived plasma cells, we demonstrated long-lived cells secreting Plasmodium-specific IgG in both bone marrow and in spleens of infected mice. The presence of these long-lived cells was independent of the presence of chronic infection, as removal of parasites with anti-malarial drugs had no impact on their numbers. Thus, in this model of malaria, both functional Plasmodium-specific memory B cells and long-lived plasma cells can be generated, suggesting that defects in generating these cell populations may not be the reason for generating short-lived antibody responses. © 2009 Ndungo et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC2784955/bin/ppat.1000690.s001.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC2784955/bin/ppat.1000690.s002.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC2784955/bin/ppat.1000690.s003.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC2784955/bin/ppat.1000690.s004.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC2784955/bin/ppat.1000690.s005.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC2784955/bin/ppat.1000690.s006.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC2784955/bin/ppat.1000690.s007.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC2784955/bin/ppat.1000690.s008.tif
Authors & Co-Authors
Ndung'u, Francis M.
United Kingdom, London
Mrc National Institute for Medical Research
Kenya, Nairobi
Wellcome Trust Research Laboratories Nairobi
Cadman, Emma Tamsin
United Kingdom, London
Mrc National Institute for Medical Research
United Kingdom, London
Royal Veterinary College University of London
Coulcher, Joshua
United Kingdom, London
Mrc National Institute for Medical Research
Nduati, Eunice Wambui
United Kingdom, London
Mrc National Institute for Medical Research
Kenya, Nairobi
Wellcome Trust Research Laboratories Nairobi
Couper, Elisabeth
United Kingdom, London
Mrc National Institute for Medical Research
MacDonald, Douglas William
United Kingdom, London
Mrc National Institute for Medical Research
Ng, Dorothy
United Kingdom, London
Mrc National Institute for Medical Research
Langhorne, Jean
United Kingdom, London
Mrc National Institute for Medical Research
Statistics
Citations: 80
Authors: 8
Affiliations: 3
Identifiers
Doi:
10.1371/journal.ppat.1000690
ISSN:
15537366
e-ISSN:
15537374
Research Areas
Infectious Diseases