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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Two Sinorhizobium meliloti glutaredoxins regulate iron metabolism and symbiotic bacteroid differentiation
Environmental Microbiology, Volume 15, No. 3, Year 2013
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Description
Summary: Legumes interact symbiotically with bacteria of the Rhizobiaceae to form nitrogen-fixing root nodules. We investigated the contribution of the three glutaredoxin (Grx)-encoding genes present in the Sinorhizobium meliloti genome to this symbiosis. SmGRX1 (CGYC active site) and SmGRX3 (CPYG) recombinant proteins displayed deglutathionylation activity in the 2-hydroethyldisulfide assay, whereas SmGRX2 (CGFS) did not. Mutation of SmGRX3 did not affect S.meliloti growth or symbiotic capacities. In contrast, SmGRX1 and SmGRX2 mutations decreased the growth of free-living bacteria and the nitrogen fixation capacity of bacteroids. Mutation of SmGRX1 led to nodule abortion and an absence of bacteroid differentiation, whereas SmGRX2 mutation decreased nodule development without modifying bacteroid development. The higher sensitivity of the Smgrx1 mutant strain as compared with wild-type strain to oxidative stress was associated with larger amounts of glutathionylated proteins. The Smgrx2 mutant strain displayed significantly lower levels of activity than the wild type for two iron-sulfur-containing enzymes, aconitase and succinate dehydrogenase. This lower level of activity could be associated with deregulation of the transcriptional activity of the RirA iron regulator and higher intracellular iron content. Thus, two S.meliloti Grx proteins are essential for symbiotic nitrogen fixation, playing independent roles in bacterial differentiation and the regulation of iron metabolism. © 2012 Society for Applied Microbiology and Blackwell Publishing Ltd.
Authors & Co-Authors
Benyamina, Sofiane M.
France, Sophia Antipolis
Institut Sophia Agrobiotech - Isa
Baldacci-Cresp, Fabien
France, Sophia Antipolis
Institut Sophia Agrobiotech - Isa
Couturier, Jérémy
France, Nancy
Université de Lorraine
Chibani, Kamel
France, Nancy
Université de Lorraine
Hopkins, Julie
France, Sophia Antipolis
Institut Sophia Agrobiotech - Isa
Bekki, Abdelkader
Algeria, Oran
Université Oran 1
de Lajudie, Philippe M.
France, Montpellier
Université de Montpellier
Rouhier, Nicolas
France, Nancy
Université de Lorraine
Jacquot, Jean-Pierre
France, Nancy
Université de Lorraine
Alloing, Geneviève
France, Sophia Antipolis
Institut Sophia Agrobiotech - Isa
Puppo, Alain
France, Sophia Antipolis
Institut Sophia Agrobiotech - Isa
Frendo, Pierre
France, Sophia Antipolis
Institut Sophia Agrobiotech - Isa
Statistics
Citations: 38
Authors: 12
Affiliations: 4
Identifiers
Doi:
10.1111/j.1462-2920.2012.02835.x
ISSN:
14622912
Research Areas
Cancer
Sexual And Reproductive Health